The Chromatin-Associated Protein PWO1 Interacts with Plant Nuclear Lamin-like Components to Regulate Nuclear Size

Pawel Mikulski, Mareike L. Hohenstatt, Sara Farrona, Cezary Smaczniak, Yvonne Stahl, Kalyanikrishna, Kerstin Kaufmann, Gerco Angenent, Daniel Schubert*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Spatial organization of chromatin contributes to gene regulation of many cellular processes and includes a connection of chromatin with the nuclear lamina (NL). The NL is a protein mesh that resides underneath the inner nuclear membrane and consists of lamins and lamina-associated proteins. Chromatin regions associated with lamins in animals are characterized mostly by constitutive heterochromatin, but association with facultative heterochromatin mediated by Polycomb-group (PcG) proteins has been reported as well. In contrast with animals, plant NL components are largely not conserved and NL association with chromatin is poorly explored. Here, we present the connection between the lamin-like protein, CROWDED NUCLEI1 (CRWN1), and the chromatin- and PcG-associated component, PROLINE-TRYPTOPHANE-TRYPTOPHANE-PROLINE INTERACTOR OF POLYCOMBS1, in Arabidopsis (Arabidopsis thaliana). We show that PWO1 and CRWN1 proteins associate physically with each other, act in the same pathway to maintain nuclear morphology, and control expression of a similar set of target genes. Moreover, we demonstrate that transiently expressed PWO1 proteins form foci located partially at the subnuclear periphery. Ultimately, as CRWN1 and PWO1 are plant-specific, our results argue that plants might have developed an equivalent, rather than homologous, mechanism of linking chromatin repression and NL.

Original languageEnglish
Pages (from-to)1141-1154
Number of pages14
JournalThe Plant Cell
Volume31
Issue number5
DOIs
Publication statusPublished - 1 May 2019

Fingerprint

Lamins
Nuclear Lamina
laminae (animals)
Chromatin
chromatin
Proteins
Heterochromatin
proteins
heterochromatin
Arabidopsis
Proline
proline
Polycomb-Group Proteins
Nuclear Envelope
nuclear membrane
Genes
animals
genes
Arabidopsis thaliana

Cite this

Mikulski, P., Hohenstatt, M. L., Farrona, S., Smaczniak, C., Stahl, Y., Kalyanikrishna, ... Schubert, D. (2019). The Chromatin-Associated Protein PWO1 Interacts with Plant Nuclear Lamin-like Components to Regulate Nuclear Size. The Plant Cell, 31(5), 1141-1154. https://doi.org/10.1105/tpc.18.00663
Mikulski, Pawel ; Hohenstatt, Mareike L. ; Farrona, Sara ; Smaczniak, Cezary ; Stahl, Yvonne ; Kalyanikrishna ; Kaufmann, Kerstin ; Angenent, Gerco ; Schubert, Daniel. / The Chromatin-Associated Protein PWO1 Interacts with Plant Nuclear Lamin-like Components to Regulate Nuclear Size. In: The Plant Cell. 2019 ; Vol. 31, No. 5. pp. 1141-1154.
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abstract = "Spatial organization of chromatin contributes to gene regulation of many cellular processes and includes a connection of chromatin with the nuclear lamina (NL). The NL is a protein mesh that resides underneath the inner nuclear membrane and consists of lamins and lamina-associated proteins. Chromatin regions associated with lamins in animals are characterized mostly by constitutive heterochromatin, but association with facultative heterochromatin mediated by Polycomb-group (PcG) proteins has been reported as well. In contrast with animals, plant NL components are largely not conserved and NL association with chromatin is poorly explored. Here, we present the connection between the lamin-like protein, CROWDED NUCLEI1 (CRWN1), and the chromatin- and PcG-associated component, PROLINE-TRYPTOPHANE-TRYPTOPHANE-PROLINE INTERACTOR OF POLYCOMBS1, in Arabidopsis (Arabidopsis thaliana). We show that PWO1 and CRWN1 proteins associate physically with each other, act in the same pathway to maintain nuclear morphology, and control expression of a similar set of target genes. Moreover, we demonstrate that transiently expressed PWO1 proteins form foci located partially at the subnuclear periphery. Ultimately, as CRWN1 and PWO1 are plant-specific, our results argue that plants might have developed an equivalent, rather than homologous, mechanism of linking chromatin repression and NL.",
author = "Pawel Mikulski and Hohenstatt, {Mareike L.} and Sara Farrona and Cezary Smaczniak and Yvonne Stahl and Kalyanikrishna and Kerstin Kaufmann and Gerco Angenent and Daniel Schubert",
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Mikulski, P, Hohenstatt, ML, Farrona, S, Smaczniak, C, Stahl, Y, Kalyanikrishna, Kaufmann, K, Angenent, G & Schubert, D 2019, 'The Chromatin-Associated Protein PWO1 Interacts with Plant Nuclear Lamin-like Components to Regulate Nuclear Size', The Plant Cell, vol. 31, no. 5, pp. 1141-1154. https://doi.org/10.1105/tpc.18.00663

The Chromatin-Associated Protein PWO1 Interacts with Plant Nuclear Lamin-like Components to Regulate Nuclear Size. / Mikulski, Pawel; Hohenstatt, Mareike L.; Farrona, Sara; Smaczniak, Cezary; Stahl, Yvonne; Kalyanikrishna; Kaufmann, Kerstin; Angenent, Gerco; Schubert, Daniel.

In: The Plant Cell, Vol. 31, No. 5, 01.05.2019, p. 1141-1154.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The Chromatin-Associated Protein PWO1 Interacts with Plant Nuclear Lamin-like Components to Regulate Nuclear Size

AU - Mikulski, Pawel

AU - Hohenstatt, Mareike L.

AU - Farrona, Sara

AU - Smaczniak, Cezary

AU - Stahl, Yvonne

AU - Kalyanikrishna, null

AU - Kaufmann, Kerstin

AU - Angenent, Gerco

AU - Schubert, Daniel

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Spatial organization of chromatin contributes to gene regulation of many cellular processes and includes a connection of chromatin with the nuclear lamina (NL). The NL is a protein mesh that resides underneath the inner nuclear membrane and consists of lamins and lamina-associated proteins. Chromatin regions associated with lamins in animals are characterized mostly by constitutive heterochromatin, but association with facultative heterochromatin mediated by Polycomb-group (PcG) proteins has been reported as well. In contrast with animals, plant NL components are largely not conserved and NL association with chromatin is poorly explored. Here, we present the connection between the lamin-like protein, CROWDED NUCLEI1 (CRWN1), and the chromatin- and PcG-associated component, PROLINE-TRYPTOPHANE-TRYPTOPHANE-PROLINE INTERACTOR OF POLYCOMBS1, in Arabidopsis (Arabidopsis thaliana). We show that PWO1 and CRWN1 proteins associate physically with each other, act in the same pathway to maintain nuclear morphology, and control expression of a similar set of target genes. Moreover, we demonstrate that transiently expressed PWO1 proteins form foci located partially at the subnuclear periphery. Ultimately, as CRWN1 and PWO1 are plant-specific, our results argue that plants might have developed an equivalent, rather than homologous, mechanism of linking chromatin repression and NL.

AB - Spatial organization of chromatin contributes to gene regulation of many cellular processes and includes a connection of chromatin with the nuclear lamina (NL). The NL is a protein mesh that resides underneath the inner nuclear membrane and consists of lamins and lamina-associated proteins. Chromatin regions associated with lamins in animals are characterized mostly by constitutive heterochromatin, but association with facultative heterochromatin mediated by Polycomb-group (PcG) proteins has been reported as well. In contrast with animals, plant NL components are largely not conserved and NL association with chromatin is poorly explored. Here, we present the connection between the lamin-like protein, CROWDED NUCLEI1 (CRWN1), and the chromatin- and PcG-associated component, PROLINE-TRYPTOPHANE-TRYPTOPHANE-PROLINE INTERACTOR OF POLYCOMBS1, in Arabidopsis (Arabidopsis thaliana). We show that PWO1 and CRWN1 proteins associate physically with each other, act in the same pathway to maintain nuclear morphology, and control expression of a similar set of target genes. Moreover, we demonstrate that transiently expressed PWO1 proteins form foci located partially at the subnuclear periphery. Ultimately, as CRWN1 and PWO1 are plant-specific, our results argue that plants might have developed an equivalent, rather than homologous, mechanism of linking chromatin repression and NL.

U2 - 10.1105/tpc.18.00663

DO - 10.1105/tpc.18.00663

M3 - Article

VL - 31

SP - 1141

EP - 1154

JO - The Plant Cell

JF - The Plant Cell

SN - 1040-4651

IS - 5

ER -

Mikulski P, Hohenstatt ML, Farrona S, Smaczniak C, Stahl Y, Kalyanikrishna et al. The Chromatin-Associated Protein PWO1 Interacts with Plant Nuclear Lamin-like Components to Regulate Nuclear Size. The Plant Cell. 2019 May 1;31(5):1141-1154. https://doi.org/10.1105/tpc.18.00663