The C-terminal domain of chikungunya virus nsP2 independently governs viral RNA replication, cytopathicity, and inhibition of interferon signaling

J.J. Fros, E. van der Maten, J.M. Vlak, G.P. Pijlman

Research output: Contribution to journalArticleAcademicpeer-review

34 Citations (Scopus)

Abstract

Alphavirus nonstructural protein 2 (nsP2) has pivotal roles in viral RNA replication, host cell shutoff, and inhibition of antiviral responses. Mutations that individually rendered other alphaviruses noncytopathic were introduced into chikungunya virus nsP2. Results show that (i) nsP2 mutation P718S only in combination with KR649AA or adaptive mutation D711G allowed noncytopathic replicon RNA replication, (ii) prohibiting nsP2 nuclear localization abrogates inhibition of antiviral interferon-induced JAK-STAT signaling, and (iii) nsP2 independently affects RNA replication, cytopathicity, and JAK-STAT signaling
Original languageEnglish
Pages (from-to)10394-10400
JournalJournal of Virology
Volume87
Issue number18
DOIs
Publication statusPublished - 2013

Keywords

  • semliki-forest-virus
  • nonstructural protein-2
  • nuclear-localization
  • infection
  • transcription
  • alphaviruses
  • translation
  • expression
  • mutations
  • cells

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