The Brassicaceae-Specific EWR1 Gene Provides Resistance to Vascular Wilt Pathogens

K.A. Yadeta, D.J. Valkenburg, M. Hanemian, Y. Marco, B.P.H.J. Thomma

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Soil-borne vascular wilt diseases caused by Verticillium spp. are among the most destructive diseases worldwide in a wide range of plant species. The most effective means of controlling Verticillium wilt diseases is the use of genetic resistance. We have previously reported the identification of four activation-tagged Arabidopsis mutants which showed enhanced resistance to Verticillium wilt. Among these, one mutant also showed enhanced resistance to Ralstonia solanacearum, a bacterial vascular wilt pathogen. Cloning of the activation tag revealed an insertion upstream of gene At3g13437, which we designated as EWR1 (for Enhancer of vascular Wilt Resistance 1) that encodes a putatively secreted protein of unknown function. The search for homologs of Arabidopsis EWR1 (AtEWR1) in public databases only identified homologs within the Brassicaceae family. We subsequently cloned the EWR1 homolog from Brassica oleracea (BoEWR1) and show that over-expression in Arabidopsis results in V. dahliae resistance. Moreover, over-expression of AtEWR1 and BoEWR1 in N. benthamiana, a member of the Solanaceae family, results in V. dahliae resistance, suggesting that EWR1 homologs can be used to engineer Verticillium wilt resistance in non-Brassicaceae crops as well.
Original languageEnglish
Article numbere88230
Number of pages11
JournalPLoS ONE
Volume9
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Verticillium
Brassicaceae
vascular wilt
Pathogens
Vascular Resistance
Genes
Arabidopsis
Verticillium wilt
pathogens
Chemical activation
genes
Cloning
Ralstonia solanacearum
Solanaceae
Crops
Insertional Mutagenesis
Brassica
Vascular Diseases
Blood Vessels
Soils

Keywords

  • asymmetric interlaced pcr
  • arabidopsis-thaliana
  • verticillium wilt
  • antimicrobial peptides
  • defense peptides
  • tomato ve1
  • plant
  • dahliae
  • disease
  • proteins

Cite this

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title = "The Brassicaceae-Specific EWR1 Gene Provides Resistance to Vascular Wilt Pathogens",
abstract = "Soil-borne vascular wilt diseases caused by Verticillium spp. are among the most destructive diseases worldwide in a wide range of plant species. The most effective means of controlling Verticillium wilt diseases is the use of genetic resistance. We have previously reported the identification of four activation-tagged Arabidopsis mutants which showed enhanced resistance to Verticillium wilt. Among these, one mutant also showed enhanced resistance to Ralstonia solanacearum, a bacterial vascular wilt pathogen. Cloning of the activation tag revealed an insertion upstream of gene At3g13437, which we designated as EWR1 (for Enhancer of vascular Wilt Resistance 1) that encodes a putatively secreted protein of unknown function. The search for homologs of Arabidopsis EWR1 (AtEWR1) in public databases only identified homologs within the Brassicaceae family. We subsequently cloned the EWR1 homolog from Brassica oleracea (BoEWR1) and show that over-expression in Arabidopsis results in V. dahliae resistance. Moreover, over-expression of AtEWR1 and BoEWR1 in N. benthamiana, a member of the Solanaceae family, results in V. dahliae resistance, suggesting that EWR1 homologs can be used to engineer Verticillium wilt resistance in non-Brassicaceae crops as well.",
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author = "K.A. Yadeta and D.J. Valkenburg and M. Hanemian and Y. Marco and B.P.H.J. Thomma",
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The Brassicaceae-Specific EWR1 Gene Provides Resistance to Vascular Wilt Pathogens. / Yadeta, K.A.; Valkenburg, D.J.; Hanemian, M.; Marco, Y.; Thomma, B.P.H.J.

In: PLoS ONE, Vol. 9, No. 2, e88230, 2014.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The Brassicaceae-Specific EWR1 Gene Provides Resistance to Vascular Wilt Pathogens

AU - Yadeta, K.A.

AU - Valkenburg, D.J.

AU - Hanemian, M.

AU - Marco, Y.

AU - Thomma, B.P.H.J.

PY - 2014

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KW - disease

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