The archaeal TFIIE homologue facilitates transcription initiation by enhancing TATA-box recognition

S.D. Bell, A.B. Brinkman, J. van der Oost, S.P. Jackson

Research output: Contribution to journalArticleAcademicpeer-review

76 Citations (Scopus)

Abstract

Transcription from many archaeal promoters can be reconstituted in vitro using recombinant TATA-box binding protein (TBP) and transcription factor B (TFB)—homologues of eukaryal TBP and TFIIB—together with purified RNA polymerase (RNAP). However, all archaeal genomes sequenced to date reveal the presence of TFE, a homologue of the -subunit of the eukaryal general transcription factor, TFIIE. We show that, while TFE is not absolutely required for transcription in the reconstituted in vitro system, it nonetheless plays a stimulatory role on some promoters and under certain conditions. Mutagenesis of the TATA box or reduction of TBP concentration in transcription reactions sensitizes a promoter to TFE addition. Conversely, saturating reactions with TBP de-sensitizes promoters to TFE. These results suggest that TFE facilitates or stabilizes interactions between TBP and the TATA box.
Original languageEnglish
Pages (from-to)133-138
JournalEmbo Reports
Volume2
DOIs
Publication statusPublished - 2001

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