The Arabidopsis thaliana AAA protein CDC48A interacts in vivo with the somatic embryogenesis receptor-like kinase 1 receptor at the plasma membrane.

J.C.M. Aker; J.W. Borst; R.B. Karlova; S.C. de Vries

doi:10.1016/j.jsb.2006.03.004

The Arabidopsis thaliana AAA protein CDC48A interacts in vivo with the somatic embryogenesis receptor-like kinase 1 receptor at the plasma membrane.

J.C.M. Aker, J.W. Borst, R.B. Karlova, S.C. de Vries

Biochemistry

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Fluorescent cell division cycle (CDC)48 proteins were studied in living plant protoplasts. CDC48A and somatic embryogenesis receptor like kinase 1 (SERK1) were found to co-localize in the endoplasmatic reticulum (ER) and at the plasma membrane (PM), but not in endosomal compartments. Fluorescent lifetime imaging microscopy (FLIM) was used to detect Fo¿rster resonance energy transfer (FRET) between CrFP/YFP-tagged CDC48A and SERK1. FRET is indicative of direct protein-protein interaction. CDC48A was found to interact only with SERK1 in small areas at the PM, but not in endosomes. These findings confirm and extend our previous findings that CDC48A in plants directly interacts with SERK1

Original language	English
Pages (from-to)	62-71
Journal	Journal of Structural Biology
Volume	156
Issue number	1
DOIs	https://doi.org/10.1016/j.jsb.2006.03.004
Publication status	Published - 2006

Keywords

valosin-containing protein
atpase
ubiquitin
cell
complex
binding
fusion
nsf
p97
degradation

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10.1016/j.jsb.2006.03.004

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title = "The Arabidopsis thaliana AAA protein CDC48A interacts in vivo with the somatic embryogenesis receptor-like kinase 1 receptor at the plasma membrane.",

abstract = "Fluorescent cell division cycle (CDC)48 proteins were studied in living plant protoplasts. CDC48A and somatic embryogenesis receptor like kinase 1 (SERK1) were found to co-localize in the endoplasmatic reticulum (ER) and at the plasma membrane (PM), but not in endosomal compartments. Fluorescent lifetime imaging microscopy (FLIM) was used to detect Fo¿rster resonance energy transfer (FRET) between CrFP/YFP-tagged CDC48A and SERK1. FRET is indicative of direct protein-protein interaction. CDC48A was found to interact only with SERK1 in small areas at the PM, but not in endosomes. These findings confirm and extend our previous findings that CDC48A in plants directly interacts with SERK1",

keywords = "valosin-containing protein, atpase, ubiquitin, cell, complex, binding, fusion, nsf, p97, degradation",

author = "J.C.M. Aker and J.W. Borst and R.B. Karlova and {de Vries}, S.C.",

year = "2006",

doi = "10.1016/j.jsb.2006.03.004",

language = "English",

volume = "156",

pages = "62--71",

journal = "Journal of Structural Biology",

issn = "1047-8477",

publisher = "Elsevier",

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TY - JOUR

T1 - The Arabidopsis thaliana AAA protein CDC48A interacts in vivo with the somatic embryogenesis receptor-like kinase 1 receptor at the plasma membrane.

AU - Aker, J.C.M.

AU - Borst, J.W.

AU - Karlova, R.B.

AU - de Vries, S.C.

PY - 2006

Y1 - 2006

N2 - Fluorescent cell division cycle (CDC)48 proteins were studied in living plant protoplasts. CDC48A and somatic embryogenesis receptor like kinase 1 (SERK1) were found to co-localize in the endoplasmatic reticulum (ER) and at the plasma membrane (PM), but not in endosomal compartments. Fluorescent lifetime imaging microscopy (FLIM) was used to detect Fo¿rster resonance energy transfer (FRET) between CrFP/YFP-tagged CDC48A and SERK1. FRET is indicative of direct protein-protein interaction. CDC48A was found to interact only with SERK1 in small areas at the PM, but not in endosomes. These findings confirm and extend our previous findings that CDC48A in plants directly interacts with SERK1

AB - Fluorescent cell division cycle (CDC)48 proteins were studied in living plant protoplasts. CDC48A and somatic embryogenesis receptor like kinase 1 (SERK1) were found to co-localize in the endoplasmatic reticulum (ER) and at the plasma membrane (PM), but not in endosomal compartments. Fluorescent lifetime imaging microscopy (FLIM) was used to detect Fo¿rster resonance energy transfer (FRET) between CrFP/YFP-tagged CDC48A and SERK1. FRET is indicative of direct protein-protein interaction. CDC48A was found to interact only with SERK1 in small areas at the PM, but not in endosomes. These findings confirm and extend our previous findings that CDC48A in plants directly interacts with SERK1

KW - valosin-containing protein

KW - atpase

KW - ubiquitin

KW - cell

KW - complex

KW - binding

KW - fusion

KW - nsf

KW - p97

KW - degradation

U2 - 10.1016/j.jsb.2006.03.004

DO - 10.1016/j.jsb.2006.03.004

M3 - Article

SN - 1047-8477

VL - 156

SP - 62

EP - 71

JO - Journal of Structural Biology

JF - Journal of Structural Biology

IS - 1

ER -

The Arabidopsis thaliana AAA protein CDC48A interacts in vivo with the somatic embryogenesis receptor-like kinase 1 receptor at the plasma membrane.

Abstract

Keywords

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