TY - JOUR
T1 - The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis
AU - Yang, Chao
AU - Sofroni, Kostika
AU - Wijnker, Erik
AU - Hamamura, Yuki
AU - Carstens, Lena
AU - Harashima, Hirofumi
AU - Stolze, Sara Christina
AU - Vezon, Daniel
AU - Chelysheva, Liudmila
AU - Orban-Nemeth, Zsuzsanna
AU - Pochon, Gaëtan
AU - Nakagami, Hirofumi
AU - Schlögelhofer, Peter
AU - Grelon, Mathilde
AU - Schnittger, Arp
PY - 2020/2/3
Y1 - 2020/2/3
N2 - Meiosis is key to sexual reproduction and genetic diversity. Here, we show that the Arabidopsis cyclin-dependent kinase Cdk1/Cdk2 homolog CDKA;1 is an important regulator of meiosis needed for several aspects of meiosis such as chromosome synapsis. We identify the chromosome axis protein ASYNAPTIC 1 (ASY1), the Arabidopsis homolog of Hop1 (homolog pairing 1), essential for synaptonemal complex formation, as a target of CDKA;1. The phosphorylation of ASY1 is required for its recruitment to the chromosome axis via ASYNAPTIC 3 (ASY3), the Arabidopsis reductional division 1 (Red1) homolog, counteracting the disassembly activity of the AAA+ ATPase PACHYTENE CHECKPOINT 2 (PCH2). Furthermore, we have identified the closure motif in ASY1, typical for HORMA domain proteins, and provide evidence that the phosphorylation of ASY1 regulates the putative self-polymerization of ASY1 along the chromosome axis. Hence, the phosphorylation of ASY1 by CDKA;1 appears to be a two-pronged mechanism to initiate chromosome axis formation in meiosis.
AB - Meiosis is key to sexual reproduction and genetic diversity. Here, we show that the Arabidopsis cyclin-dependent kinase Cdk1/Cdk2 homolog CDKA;1 is an important regulator of meiosis needed for several aspects of meiosis such as chromosome synapsis. We identify the chromosome axis protein ASYNAPTIC 1 (ASY1), the Arabidopsis homolog of Hop1 (homolog pairing 1), essential for synaptonemal complex formation, as a target of CDKA;1. The phosphorylation of ASY1 is required for its recruitment to the chromosome axis via ASYNAPTIC 3 (ASY3), the Arabidopsis reductional division 1 (Red1) homolog, counteracting the disassembly activity of the AAA+ ATPase PACHYTENE CHECKPOINT 2 (PCH2). Furthermore, we have identified the closure motif in ASY1, typical for HORMA domain proteins, and provide evidence that the phosphorylation of ASY1 regulates the putative self-polymerization of ASY1 along the chromosome axis. Hence, the phosphorylation of ASY1 by CDKA;1 appears to be a two-pronged mechanism to initiate chromosome axis formation in meiosis.
KW - ASY1
KW - ASY3
KW - CDKA;1
KW - chromosome axis
KW - PCH2
U2 - 10.15252/embj.2019101625
DO - 10.15252/embj.2019101625
M3 - Article
C2 - 31556459
AN - SCOPUS:85073948731
SN - 0261-4189
VL - 39
JO - EMBO Journal
JF - EMBO Journal
IS - 3
M1 - e101625
ER -