The alpha/beta-Hydrolase Fold 3DM Database (ABHDB) as a Tool for Protein Engineering

R. Kourist, H. Jochens, S. Bartsch, R.K.P. Kuipers, S.K. Padhi, M. Gall, D. Bottcher, H.J. Joosten, U.T. Bornscheuer

Research output: Contribution to journalArticleAcademicpeer-review

103 Citations (Scopus)

Abstract

Aligning the haystack to expose the needle: The 3DM method was used to generate a comprehensive database of the a/ß-hydrolase fold enzyme superfamily. This database facilitates the analysis of structure–function relationships and enables novel insights into this superfamily to be made. In addition high-quality libraries for protein engineering can be easily designed.
Original languageEnglish
Pages (from-to)1635-1643
JournalChemBioChem
Volume11
Issue number12
DOIs
Publication statusPublished - 2010

Keywords

  • antarctica lipase-b
  • iterative saturation mutagenesis
  • ligand-binding domain
  • directed evolution
  • tertiary alcohols
  • bacillus-subtilis
  • fluorescens esterase
  • server esther
  • enantioselectivity
  • family

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    Kourist, R., Jochens, H., Bartsch, S., Kuipers, R. K. P., Padhi, S. K., Gall, M., Bottcher, D., Joosten, H. J., & Bornscheuer, U. T. (2010). The alpha/beta-Hydrolase Fold 3DM Database (ABHDB) as a Tool for Protein Engineering. ChemBioChem, 11(12), 1635-1643. https://doi.org/10.1002/cbic.201000213