The action site of carbon dioxide in relation to inhibition of ethylene production in tomato fruit

H.P.J. de Wild, P.A. Balk, E.C.A. Fernandes, H.W. Peppelenbos

Research output: Contribution to journalArticleAcademicpeer-review

28 Citations (Scopus)

Abstract

High CO2 can inhibit ethylene production of various fruit. A high level of CO2 (20 kPa) was applied to tomato fruit (Lycopersicon esculentum Mill. cv Aromata) at 18 °C for 5 days. To investigate the primary action site of CO2, we used tomato fruit at a ripening stage where feedback regulation of ethylene production was of limited importance. Feedback reactions were further prevented by a treatment with 1-methylcyclopropene (1-MCP) before exposure to high CO2. Tomatoes with and without 1-MCP pre-treatment were exposed to 0 or 20 kPa CO2. Ethylene production, 1-aminocyclopropane-1-carboxylate (ACC) content and ACC oxidase mRNA abundance were measured after 1, 2 and 5 days exposure to 0 or 20 kPa CO2. High CO2-affected LE-ACO1, LE-ACO3 and LE-ACO4 transcripts differently. Several observations show that high CO2 did not affect the ethylene receptor: (1) CO2 had a much earlier and much stronger inhibitory effect on ethylene production than 1-MCP; (2) CO2 prevented while 1-MCP stimulated ACC accumulation; (3) CO2 prevented the 1-MCP induced decrease of LE-ACO1 abundance, and inhibited the 1-MCP induced decrease of LE-ACO3 abundance. Inhibition of ethylene production together with prevention of ACC accumulation by CO2, both in fruit with and without 1-MCP pre-treatment, points to inhibition at a site before the conversion of ACC to ethylene
Original languageEnglish
Pages (from-to)273-280
JournalPostharvest Biology and Technology
Volume36
Issue number3
DOIs
Publication statusPublished - 2005

Keywords

  • internal feedback-regulation
  • 1-aminocyclopropane-1-carboxylic acid
  • differential expression
  • gene-expression
  • higher-plants
  • acc synthase
  • apple fruit
  • pear fruit
  • biosynthesis
  • oxidase

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