The 5' non-translated region of Varroa destructor virus 1 (genus Iflavirus): structure prediction and IRES activity in Lymantria dispar cells

J.R. Ongus, E.C. Roode, C.W.A. Pleij, J.M. Vlak, M.M. van Oers

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50 Citations (Scopus)

Abstract

Structure prediction of the 5' non-translated region (NTR) of four iflavirus RNAs revealed two types of potential internal ribosome entry site (IRES), which are discriminated by size and level of complexity, in this group of viruses. In contrast to the intergenic IRES of dicistroviruses, the potential 5' IRES structures of iflaviruses do not have pseudoknots. To test the activity of one of these, a bicistronic construct was made in which the 5' NTR of Varroa destructor virus 1 (VDV-1) containing a putative IRES was cloned in between two reporter genes, enhanced green fluorescent protein and firefly luciferase (Fluc). The presence of the 5' NTR of VDV-1 greatly enhanced the expression levels of the second reporter gene (Fluc) in Lymantria dispar Ld652Y cells. The 5' NTR was active in a host-specific manner, as it showed lower activity in Spodoptera frugiperda Sf21 cells and no activity in Drosophila melanogaster S2 cells
Original languageEnglish
Pages (from-to)3397-3407
JournalJournal of General Virology
Volume87
Issue number11
DOIs
Publication statusPublished - 2006

Keywords

  • internal ribosome entry
  • picorna-like-virus
  • rhopalosiphum-padi virus
  • cricket-paralysis-virus
  • nucleotide-sequence analysis
  • hepatitis-c virus
  • translation initiation
  • triatoma-virus
  • rna viruses
  • insect

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