The 5-55 single-disulphide intermediate in folding of bovine pancreatic trypsin inhibitor

N.J. Darby; C.P.M. van Mierlo; T.E. Creighton

doi:10.1016/0014-5793(91)80251-W

The 5-55 single-disulphide intermediate in folding of bovine pancreatic trypsin inhibitor

N.J. Darby, C.P.M. van Mierlo, T.E. Creighton

Research output: Contribution to journal › Article › Academic › peer-review

38 Citations (Scopus)

Abstract

An analogue of the BPTI folding intermediate that contains only the disulphide bond between Cys-5 and Cys-55 has been prepared by mutation or the other four Cys residues to Ser. On the basis of its circular dichroism and ¹H-nuclear magnetic resonance spectra and its electrophoretic mobility, this intermediate is shown to be at least partially folded at low temperatures. This probably accounts for several of the unique properties of this intermediate observed during folding.

Original language	English
Pages (from-to)	61-64
Journal	FEBS Letters
Volume	279
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(91)80251-W
Publication status	Published - 11 Feb 1991

Keywords

Bovine pancreatic trypsin inhibitor
Nuclear magnetic resonance, Circular dichroism
Protein folding: Disulphide intermediate

Access to Document

10.1016/0014-5793(91)80251-WLicence: Publisher

Cite this

@article{7a05aa201a8640b1b96e5592bbe63a4e,

title = "The 5-55 single-disulphide intermediate in folding of bovine pancreatic trypsin inhibitor",

abstract = "An analogue of the BPTI folding intermediate that contains only the disulphide bond between Cys-5 and Cys-55 has been prepared by mutation or the other four Cys residues to Ser. On the basis of its circular dichroism and 1H-nuclear magnetic resonance spectra and its electrophoretic mobility, this intermediate is shown to be at least partially folded at low temperatures. This probably accounts for several of the unique properties of this intermediate observed during folding.",

keywords = "Bovine pancreatic trypsin inhibitor, Nuclear magnetic resonance, Circular dichroism, Protein folding: Disulphide intermediate",

author = "N.J. Darby and {van Mierlo}, C.P.M. and T.E. Creighton",

year = "1991",

month = feb,

day = "11",

doi = "10.1016/0014-5793(91)80251-W",

language = "English",

volume = "279",

pages = "61--64",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - The 5-55 single-disulphide intermediate in folding of bovine pancreatic trypsin inhibitor

AU - Darby, N.J.

AU - van Mierlo, C.P.M.

AU - Creighton, T.E.

PY - 1991/2/11

Y1 - 1991/2/11

N2 - An analogue of the BPTI folding intermediate that contains only the disulphide bond between Cys-5 and Cys-55 has been prepared by mutation or the other four Cys residues to Ser. On the basis of its circular dichroism and 1H-nuclear magnetic resonance spectra and its electrophoretic mobility, this intermediate is shown to be at least partially folded at low temperatures. This probably accounts for several of the unique properties of this intermediate observed during folding.

AB - An analogue of the BPTI folding intermediate that contains only the disulphide bond between Cys-5 and Cys-55 has been prepared by mutation or the other four Cys residues to Ser. On the basis of its circular dichroism and 1H-nuclear magnetic resonance spectra and its electrophoretic mobility, this intermediate is shown to be at least partially folded at low temperatures. This probably accounts for several of the unique properties of this intermediate observed during folding.

KW - Bovine pancreatic trypsin inhibitor

KW - Nuclear magnetic resonance, Circular dichroism

KW - Protein folding: Disulphide intermediate

U2 - 10.1016/0014-5793(91)80251-W

DO - 10.1016/0014-5793(91)80251-W

M3 - Article

C2 - 1704858

AN - SCOPUS:0026088557

SN - 0014-5793

VL - 279

SP - 61

EP - 64

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

The 5-55 single-disulphide intermediate in folding of bovine pancreatic trypsin inhibitor

Abstract

Keywords

Access to Document

Fingerprint

Cite this