Testing the effects of processing on donor human Milk: Analytical methods

Eva Kontopodi*, Kasper Hettinga, Bernd Stahl, Johannes B. van Goudoever, Ruurd M. van Elburg

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)


Holder pasteurization is the current recommended method for donor human milk treatment. This method effectively eliminates most life-threatening contaminants in donor milk, but it also greatly reduces some of its biological properties. Consequently, there is a growing interest for developing novel processing methods that can ensure both microbial inactivation and a higher retention of the functional components of donor milk. Our aim was to offer a comprehensive overview of the analytical techniques available for the evaluation of such methods. To suggest an efficient workflow for the analysis of processed donor milk, a safety analytical panel as well as a nutritional value and functionality analytical panel are discussed, together with the principles, benefits, and drawbacks of the available techniques. Concluding on the suitability of a novel method requires a multifactorial approach which can be achieved by a combination of analytical targets and by using complementary assays to cross-validate the obtained results.

Original languageEnglish
Article number131413
JournalFood Chemistry
Publication statusPublished - 30 Mar 2022


  • Donor human milk
  • Functionality
  • Milk bank, Pasteurization
  • Nutritional value
  • Safety


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