Temporal dynamics of gene expression in heat-stressed Caenorhabditis elegans

K. Jovic, M.G. Sterken, Jacopo Grilli, R.P.J. Bevers, M. Rodriguez Sanchez, J.A.G. Riksen, S. Allesina, J.E. Kammenga, L.B. Snoek

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Abstract

There is considerable insight into pathways and genes associated with heat-stress conditions. Most genes involved in stress response have been identified using mutant screens or gene knockdowns. Yet, there is limited understanding of the temporal dynamics of global gene expression in stressful environments. Here, we studied global gene expression profiles during 12 hours of heat stress in the nematode C. elegans. Using a high-resolution time series of increasing stress exposures, we found a distinct shift in gene expression patterns between 3–4 hours into the stress response, separating an initially highly dynamic phase from a later relatively stagnant phase. This turning point in expression dynamics coincided with a phenotypic turning point, as shown by a strong decrease in movement, survival and, progeny count in the days following the stress. Both detectable at transcriptional and phenotypic level, this study pin-points a relatively small time frame during heat stress at which enough damage is accumulated, making it impossible to recover the next few days.
Original languageEnglish
Article numbere0189445
Number of pages16
JournalPLoS ONE
Volume12
Issue number12
DOIs
Publication statusPublished - 11 Dec 2017

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Caenorhabditis elegans
Gene expression
heat stress
Hot Temperature
heat
Gene Expression
gene expression
stress response
Genes
Gene Knockdown Techniques
genes
pins
Transcriptome
time series analysis
Nematoda
mutants
Time series

Cite this

Jovic, K., Sterken, M. G., Grilli, J., Bevers, R. P. J., Rodriguez Sanchez, M., Riksen, J. A. G., ... Snoek, L. B. (2017). Temporal dynamics of gene expression in heat-stressed Caenorhabditis elegans. PLoS ONE, 12(12), [e0189445]. https://doi.org/10.1371/journal.pone.0189445
Jovic, K. ; Sterken, M.G. ; Grilli, Jacopo ; Bevers, R.P.J. ; Rodriguez Sanchez, M. ; Riksen, J.A.G. ; Allesina, S. ; Kammenga, J.E. ; Snoek, L.B. / Temporal dynamics of gene expression in heat-stressed Caenorhabditis elegans. In: PLoS ONE. 2017 ; Vol. 12, No. 12.
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Jovic, K, Sterken, MG, Grilli, J, Bevers, RPJ, Rodriguez Sanchez, M, Riksen, JAG, Allesina, S, Kammenga, JE & Snoek, LB 2017, 'Temporal dynamics of gene expression in heat-stressed Caenorhabditis elegans', PLoS ONE, vol. 12, no. 12, e0189445. https://doi.org/10.1371/journal.pone.0189445

Temporal dynamics of gene expression in heat-stressed Caenorhabditis elegans. / Jovic, K.; Sterken, M.G.; Grilli, Jacopo; Bevers, R.P.J.; Rodriguez Sanchez, M.; Riksen, J.A.G.; Allesina, S.; Kammenga, J.E.; Snoek, L.B.

In: PLoS ONE, Vol. 12, No. 12, e0189445, 11.12.2017.

Research output: Contribution to journalArticleAcademicpeer-review

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