TEMPO/NaClO2/NaOCl oxidation of arabinoxylans

Carolina O. Pandeirada, Donny W.H. Merkx, Hans Gerd Janssen, Yvonne Westphal, Henk A. Schols*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)


TEMPO-oxidation of neutral polysaccharides has been used to obtain polyuronides displaying improved functional properties. Although arabinoxylans (AX) from different sources may yield polyuronides with diverse properties due to their variable arabinose (Araf) substitution patterns, information of the TEMPO-oxidation of AX on its structure remains scarce. We oxidized AX using various TEMPO:NaClO2:NaOCl ratios. A TEMPO:NaClO2:NaOCl ratio of 1.0:2.6:0.4 per mol of Ara gave an oxidized-AX with high molecular weight, minimal effect on xylose appearance, and comprising charged side chains. Although NMR analyses unveiled arabinuronic acid (AraAf) as the only oxidation product in the oxidized-AX, accurate AraA quantification is still challenging. Linkage analysis showed that > 75 % of the β-(1→4)-xylan backbone remained single-substituted at position O-3 of Xyl similarly to native AX. TEMPO-oxidation of AX can be considered a promising approach to obtain arabinuronoxylans with a substitution pattern resembling its parental AX.

Original languageEnglish
Article number117781
Pages (from-to)1-10
Number of pages10
JournalCarbohydrate Polymers
Publication statusPublished - 1 May 2021


  • Arabinoxylan
  • Arabinuronic acid
  • Arabinuronoxylan
  • TEMPO-oxidation


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