Temperature and presence of ethanol affect accumulation of intracellular trehalose in Lactobacillus plantarum WCFS1 upon pulsed electric field treatment

E.M.J. Vaessen; H.A. Kemme; R.A.H. Timmermans; M.A.I. Schutyser; H.M.W. den Besten

doi:10.1016/j.bioelechem.2020.107680

Temperature and presence of ethanol affect accumulation of intracellular trehalose in Lactobacillus plantarum WCFS1 upon pulsed electric field treatment

E.M.J. Vaessen, H.A. Kemme, R.A.H. Timmermans, M.A.I. Schutyser, H.M.W. den Besten^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

Pulsed electric field (PEF) treatment can be used to increase intracellular small molecule concentrations in bacteria, which can lead to enhanced robustness of these cells during further processing. In this study we investigated the effects of the PEF treatment temperature and the presence of 8% (v/v) ethanol in the PEF medium on cell survival, membrane fluidity and intracellular trehalose concentrations of Lactobacillus plantarum WCFS1. A moderate PEF treatment temperature of 21 °C resulted in a high cell survival combined with higher intracellular trehalose concentrations compared to a treatment at 10 and 35 °C. Interestingly, highest intracellular trehalose concentrations were observed upon supplementing the PEF medium with 8% ethanol, which resulted in more than a doubling in intracellular trehalose concentrations, while culture survival was retained. Overall, this study shows that treatment temperature and PEF medium optimization are important directions for improving molecule uptake upon PEF processing.

Original language	English
Article number	107680
Journal	Bioelectrochemistry
Volume	137
Early online date	1 Oct 2020
DOIs	https://doi.org/10.1016/j.bioelechem.2020.107680
Publication status	Published - Feb 2021

Keywords

Ethanol
Intracellular trehalose
Membrane fluidity
Pulsed electric field
Temperature

Access to Document

10.1016/j.bioelechem.2020.107680Licence: CC BY-NC-ND

https://edepot.wur.nl/533587Licence: CC BY-NC-ND

Cite this

@article{e921d7e9cf6d4886a6ef7919d6043c27,

title = "Temperature and presence of ethanol affect accumulation of intracellular trehalose in Lactobacillus plantarum WCFS1 upon pulsed electric field treatment",

abstract = "Pulsed electric field (PEF) treatment can be used to increase intracellular small molecule concentrations in bacteria, which can lead to enhanced robustness of these cells during further processing. In this study we investigated the effects of the PEF treatment temperature and the presence of 8% (v/v) ethanol in the PEF medium on cell survival, membrane fluidity and intracellular trehalose concentrations of Lactobacillus plantarum WCFS1. A moderate PEF treatment temperature of 21 °C resulted in a high cell survival combined with higher intracellular trehalose concentrations compared to a treatment at 10 and 35 °C. Interestingly, highest intracellular trehalose concentrations were observed upon supplementing the PEF medium with 8% ethanol, which resulted in more than a doubling in intracellular trehalose concentrations, while culture survival was retained. Overall, this study shows that treatment temperature and PEF medium optimization are important directions for improving molecule uptake upon PEF processing.",

keywords = "Ethanol, Intracellular trehalose, Membrane fluidity, Pulsed electric field, Temperature",

author = "E.M.J. Vaessen and H.A. Kemme and R.A.H. Timmermans and M.A.I. Schutyser and {den Besten}, H.M.W.",

year = "2021",

month = feb,

doi = "10.1016/j.bioelechem.2020.107680",

language = "English",

volume = "137",

journal = "Bioelectrochemistry",

issn = "1567-5394",

publisher = "Elsevier",

}

TY - JOUR

T1 - Temperature and presence of ethanol affect accumulation of intracellular trehalose in Lactobacillus plantarum WCFS1 upon pulsed electric field treatment

AU - Vaessen, E.M.J.

AU - Kemme, H.A.

AU - Timmermans, R.A.H.

AU - Schutyser, M.A.I.

AU - den Besten, H.M.W.

PY - 2021/2

Y1 - 2021/2

N2 - Pulsed electric field (PEF) treatment can be used to increase intracellular small molecule concentrations in bacteria, which can lead to enhanced robustness of these cells during further processing. In this study we investigated the effects of the PEF treatment temperature and the presence of 8% (v/v) ethanol in the PEF medium on cell survival, membrane fluidity and intracellular trehalose concentrations of Lactobacillus plantarum WCFS1. A moderate PEF treatment temperature of 21 °C resulted in a high cell survival combined with higher intracellular trehalose concentrations compared to a treatment at 10 and 35 °C. Interestingly, highest intracellular trehalose concentrations were observed upon supplementing the PEF medium with 8% ethanol, which resulted in more than a doubling in intracellular trehalose concentrations, while culture survival was retained. Overall, this study shows that treatment temperature and PEF medium optimization are important directions for improving molecule uptake upon PEF processing.

AB - Pulsed electric field (PEF) treatment can be used to increase intracellular small molecule concentrations in bacteria, which can lead to enhanced robustness of these cells during further processing. In this study we investigated the effects of the PEF treatment temperature and the presence of 8% (v/v) ethanol in the PEF medium on cell survival, membrane fluidity and intracellular trehalose concentrations of Lactobacillus plantarum WCFS1. A moderate PEF treatment temperature of 21 °C resulted in a high cell survival combined with higher intracellular trehalose concentrations compared to a treatment at 10 and 35 °C. Interestingly, highest intracellular trehalose concentrations were observed upon supplementing the PEF medium with 8% ethanol, which resulted in more than a doubling in intracellular trehalose concentrations, while culture survival was retained. Overall, this study shows that treatment temperature and PEF medium optimization are important directions for improving molecule uptake upon PEF processing.

KW - Ethanol

KW - Intracellular trehalose

KW - Membrane fluidity

KW - Pulsed electric field

KW - Temperature

U2 - 10.1016/j.bioelechem.2020.107680

DO - 10.1016/j.bioelechem.2020.107680

M3 - Article

AN - SCOPUS:85093112306

SN - 1567-5394

VL - 137

JO - Bioelectrochemistry

JF - Bioelectrochemistry

M1 - 107680

ER -

Temperature and presence of ethanol affect accumulation of intracellular trehalose in Lactobacillus plantarum WCFS1 upon pulsed electric field treatment

Abstract

Keywords

Access to Document

Fingerprint

Cite this