Targeting trichothecene biosynthetic genes

Songhong Wei; Theo van der Lee; Els Verstappen; Marga van Gent; Cees Waalwijk

doi:10.1007/978-1-4939-6707-0_11

Targeting trichothecene biosynthetic genes

Songhong Wei, Theo van der Lee, Els Verstappen, Marga van Gent, Cees Waalwijk^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

9 Citations (Scopus)

Abstract

Biosynthesis of trichothecenes requires the involvement of at least 15 genes, most of which have been targeted for PCR. Qualitative PCRs are used to assign chemotypes to individual isolates, e.g., the capacity to produce type A and/or type B trichothecenes. Many regions in the core cluster (consisting of 12 genes) including intergenic regions have been used as targets for PCR, but the most robust assays are targeted to the tri3 and tri12 genes. Quantitative PCRs, that work across trichothecene-producing members of the Fusarium head blight complex, are described along with procedures to quantify the amount of fungal biomass in wheat samples. These assays are directed to the chemotype(s) present in field samples and quantify the total fungal biomass of trichothecene-producing fungi, irrespective of their genetic identity.

Original language	English
Title of host publication	Mycotoxigenic Fungi
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press
Pages	173-189
ISBN (Electronic)	9781493967070
ISBN (Print)	9781493967056
DOIs	https://doi.org/10.1007/978-1-4939-6707-0_11
Publication status	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1542
ISSN (Print)	1064-3745

Keywords

PCR
Quantitative PCR
Trichothecenes

Access to Document

10.1007/978-1-4939-6707-0_11

AF-EU-16010 MycoKey (BO-63-101-002, BO-46-001-006, BO-33.04-003-002)
Waalwijk, C. (Project Leader)
1/01/16 → 31/12/20
Project: LVVN project

Cite this

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abstract = "Biosynthesis of trichothecenes requires the involvement of at least 15 genes, most of which have been targeted for PCR. Qualitative PCRs are used to assign chemotypes to individual isolates, e.g., the capacity to produce type A and/or type B trichothecenes. Many regions in the core cluster (consisting of 12 genes) including intergenic regions have been used as targets for PCR, but the most robust assays are targeted to the tri3 and tri12 genes. Quantitative PCRs, that work across trichothecene-producing members of the Fusarium head blight complex, are described along with procedures to quantify the amount of fungal biomass in wheat samples. These assays are directed to the chemotype(s) present in field samples and quantify the total fungal biomass of trichothecene-producing fungi, irrespective of their genetic identity.",

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author = "Songhong Wei and {van der Lee}, Theo and Els Verstappen and {van Gent}, Marga and Cees Waalwijk",

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T1 - Targeting trichothecene biosynthetic genes

AU - Wei, Songhong

AU - van der Lee, Theo

AU - Verstappen, Els

AU - van Gent, Marga

AU - Waalwijk, Cees

PY - 2017

Y1 - 2017

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AB - Biosynthesis of trichothecenes requires the involvement of at least 15 genes, most of which have been targeted for PCR. Qualitative PCRs are used to assign chemotypes to individual isolates, e.g., the capacity to produce type A and/or type B trichothecenes. Many regions in the core cluster (consisting of 12 genes) including intergenic regions have been used as targets for PCR, but the most robust assays are targeted to the tri3 and tri12 genes. Quantitative PCRs, that work across trichothecene-producing members of the Fusarium head blight complex, are described along with procedures to quantify the amount of fungal biomass in wheat samples. These assays are directed to the chemotype(s) present in field samples and quantify the total fungal biomass of trichothecene-producing fungi, irrespective of their genetic identity.

KW - PCR

KW - Quantitative PCR

KW - Trichothecenes

U2 - 10.1007/978-1-4939-6707-0_11

DO - 10.1007/978-1-4939-6707-0_11

M3 - Chapter

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T3 - Methods in Molecular Biology

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BT - Mycotoxigenic Fungi

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Targeting trichothecene biosynthetic genes

Abstract

Publication series

Keywords

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Projects

AF-EU-16010 MycoKey (BO-63-101-002, BO-46-001-006, BO-33.04-003-002)

Cite this