Targeting trichothecene biosynthetic genes

Songhong Wei, Theo van der Lee, Els Verstappen, Marga van Gent, Cees Waalwijk*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

6 Citations (Scopus)

Abstract

Biosynthesis of trichothecenes requires the involvement of at least 15 genes, most of which have been targeted for PCR. Qualitative PCRs are used to assign chemotypes to individual isolates, e.g., the capacity to produce type A and/or type B trichothecenes. Many regions in the core cluster (consisting of 12 genes) including intergenic regions have been used as targets for PCR, but the most robust assays are targeted to the tri3 and tri12 genes. Quantitative PCRs, that work across trichothecene-producing members of the Fusarium head blight complex, are described along with procedures to quantify the amount of fungal biomass in wheat samples. These assays are directed to the chemotype(s) present in field samples and quantify the total fungal biomass of trichothecene-producing fungi, irrespective of their genetic identity.
Original languageEnglish
Title of host publicationMycotoxigenic Fungi
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages173-189
ISBN (Electronic)9781493967070
ISBN (Print)9781493967056
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1542
ISSN (Print)1064-3745

Keywords

  • PCR
  • Quantitative PCR
  • Trichothecenes

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