Target selected insertional mutagenesis on chromosome IV of Arabidopsis using the En-I transposon system

E. Speulman, R. van Asperen, J. van der Laak, W.J. Stiekema, A. Pereira

    Research output: Contribution to journalArticleAcademicpeer-review

    9 Citations (Scopus)

    Abstract

    Reverse genetics using insertional mutagenesis is an efficient experimental strategy for assessing gene functions. The maize Enhancer- Inhibitor (En-I) transposable element system was used to develop an effective reverse genetics strategy in Arabidopsis based on transposons. To generate insertion mutations in a specific chromosomal region we developed a strategy for local transposition mutagenesis. A small population of 960 plants, containing independent I transpositions was used to study local mutagenesis on chromosome IV of Arabidopsis. A total of 15 genes, located on chromosome IV, were tested for I insertions and included genes identified by the European ESSA I sequencing programme. These genes were of particular interest since homologies to other genes and gene families were identified, but their exact functions were unknown. Somatic insertions were identified for all genes tested in a few specific plants. Analysis of these progeny plants over several generations revealed that the ability to generate somatic insertions in the target gene were heritable. These genotypes that show high levels of somatic insertions can be used to identify germinal insertions in the progeny. (C) 2000 Elsevier Science B.V.
    Original languageEnglish
    Pages (from-to)301-312
    JournalJournal of Biotechnology
    Volume78
    Issue number3
    DOIs
    Publication statusPublished - 2000

    Fingerprint

    Dive into the research topics of 'Target selected insertional mutagenesis on chromosome IV of Arabidopsis using the En-I transposon system'. Together they form a unique fingerprint.

    Cite this