Tandem Mass Spectrometry in Resolving Complex Gut Microbiota Functions

Carolin Kolmeder; Kaarina Lähteenmäki; Pirjo Wacklin; Annika Kotovuori; Ilja Ritamo; Jaana Mättö; Willem M. Vos; Leena Valmu

doi:10.1002/9781118960226.ch19

Tandem Mass Spectrometry in Resolving Complex Gut Microbiota Functions

Carolin Kolmeder^*, Kaarina Lähteenmäki, Pirjo Wacklin, Annika Kotovuori, Ilja Ritamo, Jaana Mättö, Willem M. Vos, Leena Valmu

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

1 Citation (Scopus)

Abstract

This chapter reviews the technological development of high-resolution tandem mass spectrometry (MS) and its capability in analysis of complex microbial systems, particularly in the human gastrointestinal (GI) tract, and presents a few relevant examples of the metaproteomic approach. Protein MS became possible due to the rather simultaneous inventions of matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) and is now adopted as a widely used tool in identification as well as characterization of microbiota. These innovations lead to the fast development of proteomics and raised tremendous expectations for the clinical application of proteomic research. The chapter also reviews on studies addressing all the proteins in fecal samples are provided. It describes a more targeted approach that focuses on surface proteins found in intestinal microbiomes. The chapter then focuses on peptide spectral matching (PSM) and de novo sequencing.

Original language	English
Title of host publication	MALDI-TOF and Tandem MS for Clinical Microbiology
Editors	H.N. Shah, S.E. Gharbia
Publisher	Wiley
Pages	505-528
Number of pages	24
ISBN (Electronic)	9781118960226
ISBN (Print)	9781118960257
DOIs	https://doi.org/10.1002/9781118960226.ch19
Publication status	Published - 15 Sept 2016

Keywords

Complex gut microbiota functions
De novo sequencing
Electrospray ionization
Human gastrointestinal tract
Intestinal microbiomes
MALDI-TOF systems
Metaproteomic approach
Peptide spectral matching
Protein digestion
Tandem mass spectrometry

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10.1002/9781118960226.ch19

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title = "Tandem Mass Spectrometry in Resolving Complex Gut Microbiota Functions",

abstract = "This chapter reviews the technological development of high-resolution tandem mass spectrometry (MS) and its capability in analysis of complex microbial systems, particularly in the human gastrointestinal (GI) tract, and presents a few relevant examples of the metaproteomic approach. Protein MS became possible due to the rather simultaneous inventions of matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) and is now adopted as a widely used tool in identification as well as characterization of microbiota. These innovations lead to the fast development of proteomics and raised tremendous expectations for the clinical application of proteomic research. The chapter also reviews on studies addressing all the proteins in fecal samples are provided. It describes a more targeted approach that focuses on surface proteins found in intestinal microbiomes. The chapter then focuses on peptide spectral matching (PSM) and de novo sequencing.",

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AU - Kolmeder, Carolin

AU - Lähteenmäki, Kaarina

AU - Wacklin, Pirjo

AU - Kotovuori, Annika

AU - Ritamo, Ilja

AU - Mättö, Jaana

AU - Vos, Willem M.

AU - Valmu, Leena

PY - 2016/9/15

Y1 - 2016/9/15

N2 - This chapter reviews the technological development of high-resolution tandem mass spectrometry (MS) and its capability in analysis of complex microbial systems, particularly in the human gastrointestinal (GI) tract, and presents a few relevant examples of the metaproteomic approach. Protein MS became possible due to the rather simultaneous inventions of matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) and is now adopted as a widely used tool in identification as well as characterization of microbiota. These innovations lead to the fast development of proteomics and raised tremendous expectations for the clinical application of proteomic research. The chapter also reviews on studies addressing all the proteins in fecal samples are provided. It describes a more targeted approach that focuses on surface proteins found in intestinal microbiomes. The chapter then focuses on peptide spectral matching (PSM) and de novo sequencing.

AB - This chapter reviews the technological development of high-resolution tandem mass spectrometry (MS) and its capability in analysis of complex microbial systems, particularly in the human gastrointestinal (GI) tract, and presents a few relevant examples of the metaproteomic approach. Protein MS became possible due to the rather simultaneous inventions of matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) and is now adopted as a widely used tool in identification as well as characterization of microbiota. These innovations lead to the fast development of proteomics and raised tremendous expectations for the clinical application of proteomic research. The chapter also reviews on studies addressing all the proteins in fecal samples are provided. It describes a more targeted approach that focuses on surface proteins found in intestinal microbiomes. The chapter then focuses on peptide spectral matching (PSM) and de novo sequencing.

KW - Complex gut microbiota functions

KW - De novo sequencing

KW - Electrospray ionization

KW - Human gastrointestinal tract

KW - Intestinal microbiomes

KW - MALDI-TOF systems

KW - Metaproteomic approach

KW - Peptide spectral matching

KW - Protein digestion

KW - Tandem mass spectrometry

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DO - 10.1002/9781118960226.ch19

M3 - Chapter

SN - 9781118960257

SP - 505

EP - 528

BT - MALDI-TOF and Tandem MS for Clinical Microbiology

A2 - Shah, H.N.

A2 - Gharbia, S.E.

PB - Wiley

ER -

Tandem Mass Spectrometry in Resolving Complex Gut Microbiota Functions

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Keywords

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