Tandem Mass Spectrometry in Resolving Complex Gut Microbiota Functions

Carolin Kolmeder*, Kaarina Lähteenmäki, Pirjo Wacklin, Annika Kotovuori, Ilja Ritamo, Jaana Mättö, Willem M. Vos, Leena Valmu

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This chapter reviews the technological development of high-resolution tandem mass spectrometry (MS) and its capability in analysis of complex microbial systems, particularly in the human gastrointestinal (GI) tract, and presents a few relevant examples of the metaproteomic approach. Protein MS became possible due to the rather simultaneous inventions of matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) and is now adopted as a widely used tool in identification as well as characterization of microbiota. These innovations lead to the fast development of proteomics and raised tremendous expectations for the clinical application of proteomic research. The chapter also reviews on studies addressing all the proteins in fecal samples are provided. It describes a more targeted approach that focuses on surface proteins found in intestinal microbiomes. The chapter then focuses on peptide spectral matching (PSM) and de novo sequencing.

Original languageEnglish
Title of host publicationMALDI-TOF and Tandem MS for Clinical Microbiology
EditorsH.N. Shah, S.E. Gharbia
PublisherWiley
Pages505-528
Number of pages24
ISBN (Electronic)9781118960226
ISBN (Print)9781118960257
DOIs
Publication statusPublished - 15 Sep 2016

Keywords

  • Complex gut microbiota functions
  • De novo sequencing
  • Electrospray ionization
  • Human gastrointestinal tract
  • Intestinal microbiomes
  • MALDI-TOF systems
  • Metaproteomic approach
  • Peptide spectral matching
  • Protein digestion
  • Tandem mass spectrometry

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