Tagging Pathogenicity genes in Fusarium graminearum using the transposon system mimp/impala (Session 4: Pathogenesis and Plant Pathology)

M. Dufresne, T.A.J. van der Lee, S. Ben M'Barek, X. Xu, X. Zhang, T. Liu, W. Zhang, G.H.J. Kema, M.J. Daboussi, C. Waalwijk

Research output: Chapter in Book/Report/Conference proceedingConference paper

Abstract

Transposon mutagenesis was applied to generate mutants in Fusarium graminearum. The mimp1/impala system originally identified in F. oxysporum proved very promising for mutagenesis as the transposon and reinserted at high frequency in (the vicinity) of genes. A collection of mutants was screened for growth, for pathogenicity and for perithecia production.Several mutants blocked in one or more functions were obtained. The wild-type phenotype of one such mutant could be restored by complementation with a non-disrupted copy of the gene. In addition reinsertions occurred on each of the four chromosomes of F. graminearum, making this system a powerful tool in the functional analyses of the > 10,000 genes predicted in the F. graminearum genome
Original languageEnglish
Title of host publication3rd International Symposium on Fusarium Head Blight, Szeged, Hungary, 2-7 September 2008
EditorsZ. Kertisz
Place of PublicationBudapest, Hungary
PublisherAkadémiai Kiadó
Pages415-419
DOIs
Publication statusPublished - 2008
Event3rd International Symposium on Fusarium Head Blight, Szeged, Hungary -
Duration: 2 Sep 20087 Sep 2008

Publication series

NameCereal Research Communications
NumberSupplement 6
Volume36
ISSN (Print)0133-3720
ISSN (Electronic)1788-9170

Conference

Conference3rd International Symposium on Fusarium Head Blight, Szeged, Hungary
Period2/09/087/09/08

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  • Cite this

    Dufresne, M., van der Lee, T. A. J., Ben M'Barek, S., Xu, X., Zhang, X., Liu, T., Zhang, W., Kema, G. H. J., Daboussi, M. J., & Waalwijk, C. (2008). Tagging Pathogenicity genes in Fusarium graminearum using the transposon system mimp/impala (Session 4: Pathogenesis and Plant Pathology). In Z. Kertisz (Ed.), 3rd International Symposium on Fusarium Head Blight, Szeged, Hungary, 2-7 September 2008 (pp. 415-419). (Cereal Research Communications; Vol. 36, No. Supplement 6). Akadémiai Kiadó. https://doi.org/10.1556/CRC.36.2008.Suppl.B.34