Transposon mutagenesis was applied to generate mutants in Fusarium graminearum.
The mimp1/impala system originally identified in F. oxysporum proved very promising for
mutagenesis as the transposon and reinserted at high frequency in (the vicinity) of genes. A collection of mutants was screened for growth, for pathogenicity and for perithecia production.Several mutants blocked in one or more functions were obtained. The wild-type phenotype of one such mutant could be restored by complementation with a non-disrupted copy of the gene. In addition reinsertions occurred on each of the four chromosomes of F. graminearum, making this system a powerful tool in the functional analyses of the > 10,000 genes predicted in the F. graminearum genome
|Name||Cereal Research Communications|
|Conference||3rd International Symposium on Fusarium Head Blight, Szeged, Hungary|
|Period||2/09/08 → 7/09/08|