Tagging of MADS domain proteins for chromatin immunoprecipitation

S. de Folter, S.L. Urbanus, L. Zuijlen, K. Kaufmann, G.C. Angenent

    Research output: Contribution to journalArticleAcademicpeer-review

    32 Citations (Scopus)

    Abstract

    Most transcription factors fulfill their role in complexes and regulate their target genes upon binding to DNA motifs located in upstream regions or introns. To date, knowledge about transcription factor target genes and their corresponding transcription factor binding sites are still very limited. Two related methods that allow in vivo identification of transcription factor binding sites are chromatin immunoprecipitation (ChIP) and chromatin affinity purification (ChAP). For ChAP, the protein of interest is tagged with a peptide or protein, which can be used for affinity purification of the protein-DNA complex and hence, the identification of the target gene.
    Original languageEnglish
    Pages (from-to)7-47
    JournalBMC Plant Biology
    DOIs
    Publication statusPublished - 2007

    Keywords

    • floral homeotic gene
    • arabidopsis fruit-development
    • box gene
    • recombinant proteins
    • regulatory elements
    • negative regulation
    • ectopic expression
    • flower development
    • organ identity
    • purification

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