Synthesis of LewisX epitopes on proteins in plants

G.J.A. Rouwendal (Inventor), H.J. Bosch (Inventor), D.E.A. Florack (Inventor)

Research output: PatentOther research output

Abstract

The invention comprises a method to produce a Lewis X epitope on N-linked glycans on glycoproteins in a plant, plant part or plant cells, comprising expression of a nucleotide sequence encoding a galactosyltransferase, which catalyzes the addition of galactose in ßl,4-linkage to N-linked glycans, and expression of a nucleotide sequence encoding a fucosy transferase, which catalyzes the addition of fucose in al,3-linkage to the N-acetylglucosamine of GalBl-4GlcNAc structures on N-linked glycans, and expression of one or more nucleotide sequences encoding one or more glycoproteins, which are able to be provided with an N-linked glycan, in said plant, plant part or plant cell. The fucosyltransferase comprises preferably the catalyticaal active domain of FUT9 from T. nigrovi
Original languageEnglish
Patent numberWO2007145523
Priority date20/06/06
Publication statusPublished - 21 Dec 2007

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