Synthesis of Lewis X epitopes on plant N-glycans

G.J.A. Rouwendal, D.E.A. Florack, T. Hesselink, J.H.G. Cordewener, J.P.F.G. Helsper, H.J. Bosch

    Research output: Contribution to journalArticleAcademicpeer-review

    15 Citations (Scopus)

    Abstract

    Glycoproteins from tobacco line xFxG1, in which expression of a hybrid ß-(1¿4)-galactosyltransferase (GalT) and a hybrid a-(1¿3)-fucosyltransferase IXa (FUT9a) is combined, contained an abundance of hybrid N-glycans with Lewis X (LeX) epitopes. A comparison with N-glycan profiles from plants expressing only the hybrid ß-(1¿4)-galactosyltransferase suggested that the fucosylation of the LacNAc residues in line xFxG1 protected galactosylated N-glycans from endogenous plant ß-galactosidase activity
    Original languageEnglish
    Pages (from-to)1487-1493
    JournalCarbohydrate Research : an international journal
    Volume344
    Issue number12
    DOIs
    Publication statusPublished - 2009

    Keywords

    • dc-sign
    • fuc-tix
    • carbohydrate epitopes
    • beta-galactosidases
    • schistosoma-mansoni
    • molecular-cloning
    • transgenic plants
    • expression
    • antigen
    • alpha-1,3-fucosyl-transferase

    Fingerprint

    Dive into the research topics of 'Synthesis of Lewis X epitopes on plant N-glycans'. Together they form a unique fingerprint.

    Cite this