Synthesis of benzalacetone/raspberry ketone by chalcone synthase

M.J. Beekwilder (Inventor), O. Sibbensen (Inventor), J.D. Mikkelsen (Inventor), I.M. van der Meer (Inventor), R.D. Hall (Inventor), I. Qvist (Inventor)

Research output: PatentOther research output

Abstract

A host cell comprising a chalcone synthase (CHS) polypeptide sequence and a 4-coumarate CoA: ligase (4CL) sequence in which one or both are heterologous to the cell. A method of enabling benzalacetone synthase activity of a CHS protein comprising exposing the CHS to a microbial cellular environment, preferably an E. coli cell. The benzalacetone may be reduced by benzalacetone reductase (BAR) in the bacterial cell to make raspberry ketone (pHBD). The host cell may be supplied with a raspberry ketone precursor, preferably benzalacetone or p-coumaric acid. Sequences of raspberry CHS, tobacco 4CL and raspberry BAR genes and polypeptides are described as are vectors comprising CHS and 4CL sequences, preferably in combination with a PAL (phenylalanine ammonia-lyase) gene or a cinnamic acid 4-hydroxylase (C4H) gene. The invention is based on the surprising discovery that chalcone synthase has benzalacetone synthase (BAS) activity.
Original languageEnglish
Patent numberGB2416770
Publication statusPublished - 8 Feb 2007

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Oxidoreductases
Phenylalanine Ammonia-Lyase
Genes
Peptides
Mixed Function Oxygenases
Tobacco
raspberry ketone
flavanone synthetase
benzylideneacetone
Escherichia coli
4-coumarate-CoA ligase
Proteins
Rubus
4-coumaric acid
cinnamic acid

Cite this

Beekwilder, M. J., Sibbensen, O., Mikkelsen, J. D., van der Meer, I. M., Hall, R. D., & Qvist, I. (2007). Synthesis of benzalacetone/raspberry ketone by chalcone synthase. (Patent No. GB2416770).
Beekwilder, M.J. (Inventor) ; Sibbensen, O. (Inventor) ; Mikkelsen, J.D. (Inventor) ; van der Meer, I.M. (Inventor) ; Hall, R.D. (Inventor) ; Qvist, I. (Inventor). / Synthesis of benzalacetone/raspberry ketone by chalcone synthase. Patent No.: GB2416770.
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abstract = "A host cell comprising a chalcone synthase (CHS) polypeptide sequence and a 4-coumarate CoA: ligase (4CL) sequence in which one or both are heterologous to the cell. A method of enabling benzalacetone synthase activity of a CHS protein comprising exposing the CHS to a microbial cellular environment, preferably an E. coli cell. The benzalacetone may be reduced by benzalacetone reductase (BAR) in the bacterial cell to make raspberry ketone (pHBD). The host cell may be supplied with a raspberry ketone precursor, preferably benzalacetone or p-coumaric acid. Sequences of raspberry CHS, tobacco 4CL and raspberry BAR genes and polypeptides are described as are vectors comprising CHS and 4CL sequences, preferably in combination with a PAL (phenylalanine ammonia-lyase) gene or a cinnamic acid 4-hydroxylase (C4H) gene. The invention is based on the surprising discovery that chalcone synthase has benzalacetone synthase (BAS) activity.",
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Beekwilder, MJ, Sibbensen, O, Mikkelsen, JD, van der Meer, IM, Hall, RD & Qvist, I 2007, Synthesis of benzalacetone/raspberry ketone by chalcone synthase, Patent No. GB2416770.

Synthesis of benzalacetone/raspberry ketone by chalcone synthase. / Beekwilder, M.J. (Inventor); Sibbensen, O. (Inventor); Mikkelsen, J.D. (Inventor); van der Meer, I.M. (Inventor); Hall, R.D. (Inventor); Qvist, I. (Inventor).

Patent No.: GB2416770.

Research output: PatentOther research output

TY - PAT

T1 - Synthesis of benzalacetone/raspberry ketone by chalcone synthase

AU - Beekwilder, M.J.

AU - Sibbensen, O.

AU - Mikkelsen, J.D.

AU - van der Meer, I.M.

AU - Hall, R.D.

AU - Qvist, I.

PY - 2007/2/8

Y1 - 2007/2/8

N2 - A host cell comprising a chalcone synthase (CHS) polypeptide sequence and a 4-coumarate CoA: ligase (4CL) sequence in which one or both are heterologous to the cell. A method of enabling benzalacetone synthase activity of a CHS protein comprising exposing the CHS to a microbial cellular environment, preferably an E. coli cell. The benzalacetone may be reduced by benzalacetone reductase (BAR) in the bacterial cell to make raspberry ketone (pHBD). The host cell may be supplied with a raspberry ketone precursor, preferably benzalacetone or p-coumaric acid. Sequences of raspberry CHS, tobacco 4CL and raspberry BAR genes and polypeptides are described as are vectors comprising CHS and 4CL sequences, preferably in combination with a PAL (phenylalanine ammonia-lyase) gene or a cinnamic acid 4-hydroxylase (C4H) gene. The invention is based on the surprising discovery that chalcone synthase has benzalacetone synthase (BAS) activity.

AB - A host cell comprising a chalcone synthase (CHS) polypeptide sequence and a 4-coumarate CoA: ligase (4CL) sequence in which one or both are heterologous to the cell. A method of enabling benzalacetone synthase activity of a CHS protein comprising exposing the CHS to a microbial cellular environment, preferably an E. coli cell. The benzalacetone may be reduced by benzalacetone reductase (BAR) in the bacterial cell to make raspberry ketone (pHBD). The host cell may be supplied with a raspberry ketone precursor, preferably benzalacetone or p-coumaric acid. Sequences of raspberry CHS, tobacco 4CL and raspberry BAR genes and polypeptides are described as are vectors comprising CHS and 4CL sequences, preferably in combination with a PAL (phenylalanine ammonia-lyase) gene or a cinnamic acid 4-hydroxylase (C4H) gene. The invention is based on the surprising discovery that chalcone synthase has benzalacetone synthase (BAS) activity.

M3 - Patent

M1 - GB2416770

ER -

Beekwilder MJ, Sibbensen O, Mikkelsen JD, van der Meer IM, Hall RD, Qvist I, inventors. Synthesis of benzalacetone/raspberry ketone by chalcone synthase. GB2416770. 2007 Feb 8.