Synergistic action of enzyme preparations towards recalcitrant corn silage polysaccharides

K.G. Neumüller, H. Streekstra, H.A. Schols, H. Gruppen

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Corn silage, its water unextractable solids (WUS) and enzyme recalcitrant solids (ErCS) and an industrial corn silage-based anaerobic fermentation residue (AFR) represent corn substrates with different levels of recalcitrance. Compositional analysis reveals different levels of arabinoxylan substitution for WUS, ErCS and AFR, being most pronounced regarding acetic acid, glucuronic acid- and arabinose content. By screening for enzymatic degradation of WUS, ErCS and AFR, enzyme preparations exhibiting high conversion rates were identified. Furthermore significant synergistic effects were detected by blending Aspergillus niger/Talaromyces emersonii culture filtrates with various enzymes. These findings clearly highlight a necessity for a combinatorial use of enzyme preparations towards substrates with high recalcitrance characteristics to reach high degrees of degradation. Enzyme blends were identified, outperforming the individual commercial preparations. These enzyme preparations provide a basis for new, designed enzyme mixtures for corn polysaccharide degradation as a source of necessary, accessory enzyme activities.
Original languageEnglish
Pages (from-to)88-97
JournalBiomass and Bioenergy
Volume60
DOIs
Publication statusPublished - 2014

Fingerprint

silage
Polysaccharides
corn silage
polysaccharide
polysaccharides
Enzymes
maize
enzyme
enzymes
Fermentation
fermentation
Degradation
degradation
Water
substrate
glucuronic acid
arabinoxylan
corn
water
Aspergillus

Keywords

  • talaromyces-emersonii
  • wheat-straw
  • hydrolysis
  • pretreatment
  • ethanol
  • plant
  • lignocellulose
  • fermentation
  • efficiency
  • conversion

Cite this

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title = "Synergistic action of enzyme preparations towards recalcitrant corn silage polysaccharides",
abstract = "Corn silage, its water unextractable solids (WUS) and enzyme recalcitrant solids (ErCS) and an industrial corn silage-based anaerobic fermentation residue (AFR) represent corn substrates with different levels of recalcitrance. Compositional analysis reveals different levels of arabinoxylan substitution for WUS, ErCS and AFR, being most pronounced regarding acetic acid, glucuronic acid- and arabinose content. By screening for enzymatic degradation of WUS, ErCS and AFR, enzyme preparations exhibiting high conversion rates were identified. Furthermore significant synergistic effects were detected by blending Aspergillus niger/Talaromyces emersonii culture filtrates with various enzymes. These findings clearly highlight a necessity for a combinatorial use of enzyme preparations towards substrates with high recalcitrance characteristics to reach high degrees of degradation. Enzyme blends were identified, outperforming the individual commercial preparations. These enzyme preparations provide a basis for new, designed enzyme mixtures for corn polysaccharide degradation as a source of necessary, accessory enzyme activities.",
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author = "K.G. Neum{\"u}ller and H. Streekstra and H.A. Schols and H. Gruppen",
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journal = "Biomass and Bioenergy",
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Synergistic action of enzyme preparations towards recalcitrant corn silage polysaccharides. / Neumüller, K.G.; Streekstra, H.; Schols, H.A.; Gruppen, H.

In: Biomass and Bioenergy, Vol. 60, 2014, p. 88-97.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Synergistic action of enzyme preparations towards recalcitrant corn silage polysaccharides

AU - Neumüller, K.G.

AU - Streekstra, H.

AU - Schols, H.A.

AU - Gruppen, H.

PY - 2014

Y1 - 2014

N2 - Corn silage, its water unextractable solids (WUS) and enzyme recalcitrant solids (ErCS) and an industrial corn silage-based anaerobic fermentation residue (AFR) represent corn substrates with different levels of recalcitrance. Compositional analysis reveals different levels of arabinoxylan substitution for WUS, ErCS and AFR, being most pronounced regarding acetic acid, glucuronic acid- and arabinose content. By screening for enzymatic degradation of WUS, ErCS and AFR, enzyme preparations exhibiting high conversion rates were identified. Furthermore significant synergistic effects were detected by blending Aspergillus niger/Talaromyces emersonii culture filtrates with various enzymes. These findings clearly highlight a necessity for a combinatorial use of enzyme preparations towards substrates with high recalcitrance characteristics to reach high degrees of degradation. Enzyme blends were identified, outperforming the individual commercial preparations. These enzyme preparations provide a basis for new, designed enzyme mixtures for corn polysaccharide degradation as a source of necessary, accessory enzyme activities.

AB - Corn silage, its water unextractable solids (WUS) and enzyme recalcitrant solids (ErCS) and an industrial corn silage-based anaerobic fermentation residue (AFR) represent corn substrates with different levels of recalcitrance. Compositional analysis reveals different levels of arabinoxylan substitution for WUS, ErCS and AFR, being most pronounced regarding acetic acid, glucuronic acid- and arabinose content. By screening for enzymatic degradation of WUS, ErCS and AFR, enzyme preparations exhibiting high conversion rates were identified. Furthermore significant synergistic effects were detected by blending Aspergillus niger/Talaromyces emersonii culture filtrates with various enzymes. These findings clearly highlight a necessity for a combinatorial use of enzyme preparations towards substrates with high recalcitrance characteristics to reach high degrees of degradation. Enzyme blends were identified, outperforming the individual commercial preparations. These enzyme preparations provide a basis for new, designed enzyme mixtures for corn polysaccharide degradation as a source of necessary, accessory enzyme activities.

KW - talaromyces-emersonii

KW - wheat-straw

KW - hydrolysis

KW - pretreatment

KW - ethanol

KW - plant

KW - lignocellulose

KW - fermentation

KW - efficiency

KW - conversion

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DO - 10.1016/j.biombioe.2013.11.004

M3 - Article

VL - 60

SP - 88

EP - 97

JO - Biomass and Bioenergy

JF - Biomass and Bioenergy

SN - 0961-9534

ER -