Synergism in the effect of prior jasmonic acid application on herbivore-induced volatile emission by Lima bean plants: transcription of a monoterpene synthase gene and volatile emission

T.R. Menzel, B.T. Weldegergis, A. David, W. Boland, R. Gols, J.J.A. van Loon, M. Dicke

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)

Abstract

Jasmonic acid (JA) plays a central role in induced plant defence e.g. by regulating the biosynthesis of herbivore-induced plant volatiles that mediate the attraction of natural enemies of herbivores. Moreover, exogenous application of JA can be used to elicit plant defence responses similar to those induced by biting-chewing herbivores and mites that pierce cells and consume their contents. In the present study, we used Lima bean (Phaseolus lunatus) plants to explore how application of a low dose of JA followed by minor herbivory by spider mites (Tetranychus urticae) affects transcript levels of P. lunatus (E)-ß-ocimene synthase (PlOS), emission of (E)-ß-ocimene and nine other plant volatiles commonly associated with herbivory. Furthermore, we investigated the plant’s phytohormonal response. Application of a low dose of JA increased PlOS transcript levels in a synergistic manner when followed by minor herbivory for both simultaneous and sequential infestation. Emission of (E)-ß-ocimene was also increased, and only JA, but not SA, levels were affected by treatments. Projection to latent structures-discriminant analysis (PLS-DA) of other volatiles showed overlap between treatments. Thus, a low-dose JA application results in a synergistic effect on gene transcription and an increased emission of a volatile compound involved in indirect defence after herbivore infestation.
Original languageEnglish
Pages (from-to)4821-4831
JournalJournal of Experimental Botany
Volume65
Issue number17
DOIs
Publication statusPublished - 2014

Fingerprint

lima beans
Herbivory
synergism
jasmonic acid
monoterpenoids
herbivores
transcription (genetics)
ocimene
Genes
genes
Phaseolus lunatus
dosage
Tetranychidae
Phaseolus
Mites
Mastication
Tetranychus urticae
Discriminant Analysis
mastication
pinene cyclase I

Keywords

  • induced resistance
  • arabidopsis-thaliana
  • brassica-oleracea
  • phytoseiulus-persimilis
  • attract parasitoids
  • signaling pathway
  • methyl salicylate
  • predatory mite
  • corn plants
  • host-plant

Cite this

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title = "Synergism in the effect of prior jasmonic acid application on herbivore-induced volatile emission by Lima bean plants: transcription of a monoterpene synthase gene and volatile emission",
abstract = "Jasmonic acid (JA) plays a central role in induced plant defence e.g. by regulating the biosynthesis of herbivore-induced plant volatiles that mediate the attraction of natural enemies of herbivores. Moreover, exogenous application of JA can be used to elicit plant defence responses similar to those induced by biting-chewing herbivores and mites that pierce cells and consume their contents. In the present study, we used Lima bean (Phaseolus lunatus) plants to explore how application of a low dose of JA followed by minor herbivory by spider mites (Tetranychus urticae) affects transcript levels of P. lunatus (E)-{\ss}-ocimene synthase (PlOS), emission of (E)-{\ss}-ocimene and nine other plant volatiles commonly associated with herbivory. Furthermore, we investigated the plant’s phytohormonal response. Application of a low dose of JA increased PlOS transcript levels in a synergistic manner when followed by minor herbivory for both simultaneous and sequential infestation. Emission of (E)-{\ss}-ocimene was also increased, and only JA, but not SA, levels were affected by treatments. Projection to latent structures-discriminant analysis (PLS-DA) of other volatiles showed overlap between treatments. Thus, a low-dose JA application results in a synergistic effect on gene transcription and an increased emission of a volatile compound involved in indirect defence after herbivore infestation.",
keywords = "induced resistance, arabidopsis-thaliana, brassica-oleracea, phytoseiulus-persimilis, attract parasitoids, signaling pathway, methyl salicylate, predatory mite, corn plants, host-plant",
author = "T.R. Menzel and B.T. Weldegergis and A. David and W. Boland and R. Gols and {van Loon}, J.J.A. and M. Dicke",
year = "2014",
doi = "10.1093/jxb/eru242",
language = "English",
volume = "65",
pages = "4821--4831",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "17",

}

Synergism in the effect of prior jasmonic acid application on herbivore-induced volatile emission by Lima bean plants: transcription of a monoterpene synthase gene and volatile emission. / Menzel, T.R.; Weldegergis, B.T.; David, A.; Boland, W.; Gols, R.; van Loon, J.J.A.; Dicke, M.

In: Journal of Experimental Botany, Vol. 65, No. 17, 2014, p. 4821-4831.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Synergism in the effect of prior jasmonic acid application on herbivore-induced volatile emission by Lima bean plants: transcription of a monoterpene synthase gene and volatile emission

AU - Menzel, T.R.

AU - Weldegergis, B.T.

AU - David, A.

AU - Boland, W.

AU - Gols, R.

AU - van Loon, J.J.A.

AU - Dicke, M.

PY - 2014

Y1 - 2014

N2 - Jasmonic acid (JA) plays a central role in induced plant defence e.g. by regulating the biosynthesis of herbivore-induced plant volatiles that mediate the attraction of natural enemies of herbivores. Moreover, exogenous application of JA can be used to elicit plant defence responses similar to those induced by biting-chewing herbivores and mites that pierce cells and consume their contents. In the present study, we used Lima bean (Phaseolus lunatus) plants to explore how application of a low dose of JA followed by minor herbivory by spider mites (Tetranychus urticae) affects transcript levels of P. lunatus (E)-ß-ocimene synthase (PlOS), emission of (E)-ß-ocimene and nine other plant volatiles commonly associated with herbivory. Furthermore, we investigated the plant’s phytohormonal response. Application of a low dose of JA increased PlOS transcript levels in a synergistic manner when followed by minor herbivory for both simultaneous and sequential infestation. Emission of (E)-ß-ocimene was also increased, and only JA, but not SA, levels were affected by treatments. Projection to latent structures-discriminant analysis (PLS-DA) of other volatiles showed overlap between treatments. Thus, a low-dose JA application results in a synergistic effect on gene transcription and an increased emission of a volatile compound involved in indirect defence after herbivore infestation.

AB - Jasmonic acid (JA) plays a central role in induced plant defence e.g. by regulating the biosynthesis of herbivore-induced plant volatiles that mediate the attraction of natural enemies of herbivores. Moreover, exogenous application of JA can be used to elicit plant defence responses similar to those induced by biting-chewing herbivores and mites that pierce cells and consume their contents. In the present study, we used Lima bean (Phaseolus lunatus) plants to explore how application of a low dose of JA followed by minor herbivory by spider mites (Tetranychus urticae) affects transcript levels of P. lunatus (E)-ß-ocimene synthase (PlOS), emission of (E)-ß-ocimene and nine other plant volatiles commonly associated with herbivory. Furthermore, we investigated the plant’s phytohormonal response. Application of a low dose of JA increased PlOS transcript levels in a synergistic manner when followed by minor herbivory for both simultaneous and sequential infestation. Emission of (E)-ß-ocimene was also increased, and only JA, but not SA, levels were affected by treatments. Projection to latent structures-discriminant analysis (PLS-DA) of other volatiles showed overlap between treatments. Thus, a low-dose JA application results in a synergistic effect on gene transcription and an increased emission of a volatile compound involved in indirect defence after herbivore infestation.

KW - induced resistance

KW - arabidopsis-thaliana

KW - brassica-oleracea

KW - phytoseiulus-persimilis

KW - attract parasitoids

KW - signaling pathway

KW - methyl salicylate

KW - predatory mite

KW - corn plants

KW - host-plant

U2 - 10.1093/jxb/eru242

DO - 10.1093/jxb/eru242

M3 - Article

VL - 65

SP - 4821

EP - 4831

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 17

ER -