Surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry: A tool to predict pork quality

B. Marcos, P. Gou, M.D. Guardia, M. Hortos, M. Colleo, N. Mach Casellas, M.F.W. te Pas, E. Keuning, L. Kruijt, J. Tibau, M. Gispert, J. Arnau

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5 Citations (Scopus)

Abstract

Expression of water soluble proteins of fresh pork Longissimus thoracis from 4 pure breed pigs (Duroc, Large White, Landrace, and Pietrain) was studied to identify candidate protein markers for meat quality. Surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI-TOF-MS) was used to obtain the soluble protein profiles of Longissimus thoracis muscles. The pure breeds showed differences among the studied meat quality traits (pH(u), drip loss, androstenone, marbling, intramuscular fat, texture, and moisture), but no significant differences were detected in sensory analysis. Associations between protein peaks obtained with SELDI-TOF-MS and meat quality traits, mainly water holding capacity, texture and skatole were observed. Of these peaks, a total of 10 peaks from CM10 array and 6 peaks from Q10 array were candidate soluble protein markers for pork loin quality. The developed models explained a limited proportion of the variability, however they point out interesting relationships between protein expression and meat quality. (C) 2012 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)688-693
JournalMeat Science
Volume95
Issue number3
DOIs
Publication statusPublished - 2013

Keywords

  • dry-cured hams
  • seldi-tof-ms
  • boar taint
  • sensory characteristics
  • meat quality
  • androstenone
  • pigs
  • fat

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    Marcos, B., Gou, P., Guardia, M. D., Hortos, M., Colleo, M., Mach Casellas, N., te Pas, M. F. W., Keuning, E., Kruijt, L., Tibau, J., Gispert, M., & Arnau, J. (2013). Surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry: A tool to predict pork quality. Meat Science, 95(3), 688-693. https://doi.org/10.1016/j.meatsci.2012.10.014