Surface characterization and antifouling properties of nanostructured gold chips for imaging surface plasmon resonance biosensing

S. Joshi, P. Pellacani, T.A. van Beek, H. Zuilhof, M.W.F. Nielen

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

Surface Plasmon Resonance (SPR) optical sensing is a label-free technique for real-time monitoring of biomolecular interactions. Recently, a portable imaging SPR (iSPR) prototype instrument, featuring a nanostructured gold chip, has been developed. In the present work, we investigated the crucial first steps, prior to eventual use of the nanostructured iSPR chip, i.e., its surface modification, in-depth surface characterization and the antifouling performance. Results were compared with conventional flat (i)SPR gold chips having the same surface chemistries, viz. different types of polyethylene glycol and zwitterionic polymers. Characterization of the (i)SPR chips before and after surface modification was performed using atomic force microscopy (AFM), scanning electron microscopy (SEM), water contact angle (WCA), X-ray photoelectron spectroscopy (XPS) and direct analysis in real time high resolution mass spectrometry (DART-HRMS). The antifouling properties were then studied using the nanostructured chip in the portable iSPR instrument and the flat gold chip in conventional SPR setup. The zwitterionic polymer surface chemistries showed the best antifouling properties. Comparison of the nanostructured iSPR chips with conventional flat (i)SPR gold chips showed that the latter perform slightly better in terms of surface modification as well as antifouling properties. The portable iSPR instrument is almost as sensitive as conventional iSPR (IBIS) and nine times less sensitive than conventional SPR (Biacore 3000). The nanostructured iSPR chip, along with the portable instrument, offers the advantage of about ten-fold reduction in instrument size, weight and costs compared to conventional (i)SPR instruments using flat gold, thus making it highly interesting for future biosensing applications.
Original languageEnglish
Pages (from-to)505-514
JournalSensors and Actuators B: Chemical
Volume209
DOIs
Publication statusPublished - 2015

Fingerprint

antifouling
Surface plasmon resonance
surface plasmon resonance
Gold
chips
gold
Imaging techniques
Surface treatment
Surface chemistry
Polymers
chemistry
polymers
Polyethylene glycols
Contact angle
Mass spectrometry
Labels
Atomic force microscopy
X ray photoelectron spectroscopy
glycols
polyethylenes

Keywords

  • mass-spectrometry
  • nonspecific adsorption
  • zwitterionic polymers
  • organic monolayers
  • films
  • spr
  • functionalization
  • immobilization
  • microscopy
  • proteins

Cite this

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title = "Surface characterization and antifouling properties of nanostructured gold chips for imaging surface plasmon resonance biosensing",
abstract = "Surface Plasmon Resonance (SPR) optical sensing is a label-free technique for real-time monitoring of biomolecular interactions. Recently, a portable imaging SPR (iSPR) prototype instrument, featuring a nanostructured gold chip, has been developed. In the present work, we investigated the crucial first steps, prior to eventual use of the nanostructured iSPR chip, i.e., its surface modification, in-depth surface characterization and the antifouling performance. Results were compared with conventional flat (i)SPR gold chips having the same surface chemistries, viz. different types of polyethylene glycol and zwitterionic polymers. Characterization of the (i)SPR chips before and after surface modification was performed using atomic force microscopy (AFM), scanning electron microscopy (SEM), water contact angle (WCA), X-ray photoelectron spectroscopy (XPS) and direct analysis in real time high resolution mass spectrometry (DART-HRMS). The antifouling properties were then studied using the nanostructured chip in the portable iSPR instrument and the flat gold chip in conventional SPR setup. The zwitterionic polymer surface chemistries showed the best antifouling properties. Comparison of the nanostructured iSPR chips with conventional flat (i)SPR gold chips showed that the latter perform slightly better in terms of surface modification as well as antifouling properties. The portable iSPR instrument is almost as sensitive as conventional iSPR (IBIS) and nine times less sensitive than conventional SPR (Biacore 3000). The nanostructured iSPR chip, along with the portable instrument, offers the advantage of about ten-fold reduction in instrument size, weight and costs compared to conventional (i)SPR instruments using flat gold, thus making it highly interesting for future biosensing applications.",
keywords = "mass-spectrometry, nonspecific adsorption, zwitterionic polymers, organic monolayers, films, spr, functionalization, immobilization, microscopy, proteins",
author = "S. Joshi and P. Pellacani and {van Beek}, T.A. and H. Zuilhof and M.W.F. Nielen",
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language = "English",
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pages = "505--514",
journal = "Sensors and Actuators B: Chemical",
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}

Surface characterization and antifouling properties of nanostructured gold chips for imaging surface plasmon resonance biosensing. / Joshi, S.; Pellacani, P.; van Beek, T.A.; Zuilhof, H.; Nielen, M.W.F.

In: Sensors and Actuators B: Chemical, Vol. 209, 2015, p. 505-514.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Surface characterization and antifouling properties of nanostructured gold chips for imaging surface plasmon resonance biosensing

AU - Joshi, S.

AU - Pellacani, P.

AU - van Beek, T.A.

AU - Zuilhof, H.

AU - Nielen, M.W.F.

PY - 2015

Y1 - 2015

N2 - Surface Plasmon Resonance (SPR) optical sensing is a label-free technique for real-time monitoring of biomolecular interactions. Recently, a portable imaging SPR (iSPR) prototype instrument, featuring a nanostructured gold chip, has been developed. In the present work, we investigated the crucial first steps, prior to eventual use of the nanostructured iSPR chip, i.e., its surface modification, in-depth surface characterization and the antifouling performance. Results were compared with conventional flat (i)SPR gold chips having the same surface chemistries, viz. different types of polyethylene glycol and zwitterionic polymers. Characterization of the (i)SPR chips before and after surface modification was performed using atomic force microscopy (AFM), scanning electron microscopy (SEM), water contact angle (WCA), X-ray photoelectron spectroscopy (XPS) and direct analysis in real time high resolution mass spectrometry (DART-HRMS). The antifouling properties were then studied using the nanostructured chip in the portable iSPR instrument and the flat gold chip in conventional SPR setup. The zwitterionic polymer surface chemistries showed the best antifouling properties. Comparison of the nanostructured iSPR chips with conventional flat (i)SPR gold chips showed that the latter perform slightly better in terms of surface modification as well as antifouling properties. The portable iSPR instrument is almost as sensitive as conventional iSPR (IBIS) and nine times less sensitive than conventional SPR (Biacore 3000). The nanostructured iSPR chip, along with the portable instrument, offers the advantage of about ten-fold reduction in instrument size, weight and costs compared to conventional (i)SPR instruments using flat gold, thus making it highly interesting for future biosensing applications.

AB - Surface Plasmon Resonance (SPR) optical sensing is a label-free technique for real-time monitoring of biomolecular interactions. Recently, a portable imaging SPR (iSPR) prototype instrument, featuring a nanostructured gold chip, has been developed. In the present work, we investigated the crucial first steps, prior to eventual use of the nanostructured iSPR chip, i.e., its surface modification, in-depth surface characterization and the antifouling performance. Results were compared with conventional flat (i)SPR gold chips having the same surface chemistries, viz. different types of polyethylene glycol and zwitterionic polymers. Characterization of the (i)SPR chips before and after surface modification was performed using atomic force microscopy (AFM), scanning electron microscopy (SEM), water contact angle (WCA), X-ray photoelectron spectroscopy (XPS) and direct analysis in real time high resolution mass spectrometry (DART-HRMS). The antifouling properties were then studied using the nanostructured chip in the portable iSPR instrument and the flat gold chip in conventional SPR setup. The zwitterionic polymer surface chemistries showed the best antifouling properties. Comparison of the nanostructured iSPR chips with conventional flat (i)SPR gold chips showed that the latter perform slightly better in terms of surface modification as well as antifouling properties. The portable iSPR instrument is almost as sensitive as conventional iSPR (IBIS) and nine times less sensitive than conventional SPR (Biacore 3000). The nanostructured iSPR chip, along with the portable instrument, offers the advantage of about ten-fold reduction in instrument size, weight and costs compared to conventional (i)SPR instruments using flat gold, thus making it highly interesting for future biosensing applications.

KW - mass-spectrometry

KW - nonspecific adsorption

KW - zwitterionic polymers

KW - organic monolayers

KW - films

KW - spr

KW - functionalization

KW - immobilization

KW - microscopy

KW - proteins

U2 - 10.1016/j.snb.2014.11.133

DO - 10.1016/j.snb.2014.11.133

M3 - Article

VL - 209

SP - 505

EP - 514

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

SN - 0925-4005

ER -