Summary of the first round analyses of the Third International Workshop on Swine Leukocyte Differentiation Antigens

K. Haverson; A. Saalmuller; Z. Chen; C.A. Huang; A. Simon; J. Seebach; W.J.A. Boersma; R. Zwart; T.A. Niewold; E. Thacker; D. Llanes; J.M.P. de la Lastra; H. Engelhardt; A. Ezquerra; F. Alonso; J. Dominguez; J.A. Ledbetter; L. Grosmaire; R. Lee; J. Nielsen; H. Salmon; I. Valpotic; L. Sver; G. Lackovic; A. Summerfield; K.V. Khanna

doi:10.1016/S0165-2427(01)00291-4

Summary of the first round analyses of the Third International Workshop on Swine Leukocyte Differentiation Antigens

K. Haverson, A. Saalmuller, Z. Chen, C.A. Huang, A. Simon, J. Seebach, W.J.A. Boersma, R. Zwart, T.A. Niewold, E. Thacker, D. Llanes, J.M.P. de la Lastra, H. Engelhardt, A. Ezquerra, F. Alonso, J. Dominguez, J.A. Ledbetter, L. Grosmaire, R. Lee, J. NielsenH. Salmon, I. Valpotic, L. Sver, G. Lackovic, A. Summerfield, K.V. Khanna

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Abstract

The reactivity of 155 monoclonal antibodies submitted to the Third International Workshop on Swine Leukocyte Differentiation Antigens, together with 41 internal standards, was analysed by flow cytometry on 29 different pig cell targets as well as two human cell targets as a means of establishing suitable panels of monoclonal antibodies for more detailed clustering analyses by the various subsections of the workshop. Results were collected either without further gating, with gating based on FS/SS characteristics or with gating based on the co-expression of a reference antibody in two-colour flow cytometry. The CD or SWC reactivity of the internal standards had been established in previous workshops. Data sets were subsequently analysed by statistical clustering using the Leucocyte Typing Database IV software. The resulting 18 cluster groups were allocated to the appropriate second round sections of the workshop, after reviewing the overall cellular reactivity of each cluster as well as the specificity of known standards which clustered in a group.

Original language	English
Pages (from-to)	25-34
Journal	Veterinary Immunology and Immunopathology
Volume	80
Issue number	1-2
DOIs	https://doi.org/10.1016/S0165-2427(01)00291-4
Publication status	Published - 2001

Keywords

Cluster of differentiation (CD) antigens
Clustering analysis
Flow cytometry
Monoclonal antibodies (mAb)
Swine

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10.1016/S0165-2427(01)00291-4

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Haverson, K., Saalmuller, A., Chen, Z., Huang, C. A., Simon, A., Seebach, J., Boersma, W. J. A., Zwart, R., Niewold, T. A., Thacker, E., Llanes, D., de la Lastra, J. M. P., Engelhardt, H., Ezquerra, A., Alonso, F., Dominguez, J., Ledbetter, J. A., Grosmaire, L., Lee, R., ... Khanna, K. V. (2001). Summary of the first round analyses of the Third International Workshop on Swine Leukocyte Differentiation Antigens. Veterinary Immunology and Immunopathology, 80(1-2), 25-34. https://doi.org/10.1016/S0165-2427(01)00291-4

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title = "Summary of the first round analyses of the Third International Workshop on Swine Leukocyte Differentiation Antigens",

abstract = "The reactivity of 155 monoclonal antibodies submitted to the Third International Workshop on Swine Leukocyte Differentiation Antigens, together with 41 internal standards, was analysed by flow cytometry on 29 different pig cell targets as well as two human cell targets as a means of establishing suitable panels of monoclonal antibodies for more detailed clustering analyses by the various subsections of the workshop. Results were collected either without further gating, with gating based on FS/SS characteristics or with gating based on the co-expression of a reference antibody in two-colour flow cytometry. The CD or SWC reactivity of the internal standards had been established in previous workshops. Data sets were subsequently analysed by statistical clustering using the Leucocyte Typing Database IV software. The resulting 18 cluster groups were allocated to the appropriate second round sections of the workshop, after reviewing the overall cellular reactivity of each cluster as well as the specificity of known standards which clustered in a group.",

keywords = "Cluster of differentiation (CD) antigens, Clustering analysis, Flow cytometry, Monoclonal antibodies (mAb), Swine",

author = "K. Haverson and A. Saalmuller and Z. Chen and C.A. Huang and A. Simon and J. Seebach and W.J.A. Boersma and R. Zwart and T.A. Niewold and E. Thacker and D. Llanes and {de la Lastra}, J.M.P. and H. Engelhardt and A. Ezquerra and F. Alonso and J. Dominguez and J.A. Ledbetter and L. Grosmaire and R. Lee and J. Nielsen and H. Salmon and I. Valpotic and L. Sver and G. Lackovic and A. Summerfield and K.V. Khanna",

year = "2001",

doi = "10.1016/S0165-2427(01)00291-4",

language = "English",

volume = "80",

pages = "25--34",

journal = "Veterinary Immunology and Immunopathology",

issn = "0165-2427",

publisher = "Elsevier",

number = "1-2",

}

Haverson, K, Saalmuller, A, Chen, Z, Huang, CA, Simon, A, Seebach, J, Boersma, WJA, Zwart, R, Niewold, TA, Thacker, E, Llanes, D, de la Lastra, JMP, Engelhardt, H, Ezquerra, A, Alonso, F, Dominguez, J, Ledbetter, JA, Grosmaire, L, Lee, R, Nielsen, J, Salmon, H, Valpotic, I, Sver, L, Lackovic, G, Summerfield, A & Khanna, KV 2001, 'Summary of the first round analyses of the Third International Workshop on Swine Leukocyte Differentiation Antigens', Veterinary Immunology and Immunopathology, vol. 80, no. 1-2, pp. 25-34. https://doi.org/10.1016/S0165-2427(01)00291-4

TY - JOUR

T1 - Summary of the first round analyses of the Third International Workshop on Swine Leukocyte Differentiation Antigens

AU - Haverson, K.

AU - Saalmuller, A.

AU - Chen, Z.

AU - Huang, C.A.

AU - Simon, A.

AU - Seebach, J.

AU - Boersma, W.J.A.

AU - Zwart, R.

AU - Niewold, T.A.

AU - Thacker, E.

AU - Llanes, D.

AU - de la Lastra, J.M.P.

AU - Engelhardt, H.

AU - Ezquerra, A.

AU - Alonso, F.

AU - Dominguez, J.

AU - Ledbetter, J.A.

AU - Grosmaire, L.

AU - Lee, R.

AU - Nielsen, J.

AU - Salmon, H.

AU - Valpotic, I.

AU - Sver, L.

AU - Lackovic, G.

AU - Summerfield, A.

AU - Khanna, K.V.

PY - 2001

Y1 - 2001

N2 - The reactivity of 155 monoclonal antibodies submitted to the Third International Workshop on Swine Leukocyte Differentiation Antigens, together with 41 internal standards, was analysed by flow cytometry on 29 different pig cell targets as well as two human cell targets as a means of establishing suitable panels of monoclonal antibodies for more detailed clustering analyses by the various subsections of the workshop. Results were collected either without further gating, with gating based on FS/SS characteristics or with gating based on the co-expression of a reference antibody in two-colour flow cytometry. The CD or SWC reactivity of the internal standards had been established in previous workshops. Data sets were subsequently analysed by statistical clustering using the Leucocyte Typing Database IV software. The resulting 18 cluster groups were allocated to the appropriate second round sections of the workshop, after reviewing the overall cellular reactivity of each cluster as well as the specificity of known standards which clustered in a group.

AB - The reactivity of 155 monoclonal antibodies submitted to the Third International Workshop on Swine Leukocyte Differentiation Antigens, together with 41 internal standards, was analysed by flow cytometry on 29 different pig cell targets as well as two human cell targets as a means of establishing suitable panels of monoclonal antibodies for more detailed clustering analyses by the various subsections of the workshop. Results were collected either without further gating, with gating based on FS/SS characteristics or with gating based on the co-expression of a reference antibody in two-colour flow cytometry. The CD or SWC reactivity of the internal standards had been established in previous workshops. Data sets were subsequently analysed by statistical clustering using the Leucocyte Typing Database IV software. The resulting 18 cluster groups were allocated to the appropriate second round sections of the workshop, after reviewing the overall cellular reactivity of each cluster as well as the specificity of known standards which clustered in a group.

KW - Cluster of differentiation (CD) antigens

KW - Clustering analysis

KW - Flow cytometry

KW - Monoclonal antibodies (mAb)

KW - Swine

U2 - 10.1016/S0165-2427(01)00291-4

DO - 10.1016/S0165-2427(01)00291-4

M3 - Article

SN - 0165-2427

VL - 80

SP - 25

EP - 34

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 1-2

ER -

Summary of the first round analyses of the Third International Workshop on Swine Leukocyte Differentiation Antigens

Abstract

Keywords

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