Substrate-induced production and secretion of cellulases by Clostridium acetobutylicum

A.M. Lopez Contreras, K. Gabor, A.A. Martens, B.A.M. Renckens, P.A.M. Claassen, J. van der Oost, W.M. de Vos

Research output: Contribution to journalArticleAcademicpeer-review

35 Citations (Scopus)

Abstract

Clostridium acetobutylicum ATCC 824 is a solventogenic bacterium that grows heterotrophically on a variety of carbohydrates, including glucose, cellobiose, xylose, and lichenan, a linear polymer of beta-1,3- and beta-1,4-linked beta-D-glucose units. C. acetobutylicum does not degrade cellulose, although its genome sequence contains several cellulase-encoding genes and a complete cellulosome cluster of cellulosome genes. In the present study, we demonstrate that a low but significant level of induction of cellulase activity occurs during growth on xylose or lichenan. The celF gene, located in the cellulosome-like gene cluster and coding for a unique cellulase that belongs to glycoside hydrolase family 48, was cloned in Escherichia coli, and antibodies were raised against the overproduced CelF protein. A Western blot analysis suggested a possible catabolite repression by glucose or cellobiose and an up-regulation by lichenan or xylose of the extracellular production of CelF by C. acetobutylicum. Possible reasons for the apparent inability of C. acetobutylicum to degrade cellulose are discussed.
Original languageEnglish
Pages (from-to)5238-5243
JournalApplied and Environmental Microbiology
Volume70
Issue number9
DOIs
Publication statusPublished - 2004

    Fingerprint

Keywords

  • cellulose-binding domain
  • gram-positive bacteria
  • catabolite repression
  • trichoderma-reesei
  • solvent production
  • bacillus-subtilis
  • cellulovorans
  • gene
  • thermocellum
  • degradation

Cite this