Sublocalization of an invasion-inducing locus and other genes on human chromosome 7

G.G.M. Habets, R.A. Van der Kammen, V. Willemsen, M. Balemans, J. Wiegant, John G. Collard*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)

Abstract

By somatic cell fusion studies between noninvasive mouse T-lymphoma cells and invasive human activated normal T-cells we have previously shown that the genetic information responsible for the induction of invasive and metastatic potential in interspecies T-cell hybrids is located on human chromosome 7. Apparently, genes derived from normal activated T-cells are dominantly expressed in the hybrids and control the invasive and. as a consequence, metastatic potential of these T-lymphoma cells. To sublocalize the invasion-inducing locus on chromosome 7 we have generated hybrids that harbor only specific regions of human chromosome 7 with or without a small fragment of human chromosome 21. Analysis of these hybrids revealed that the invasion-inducing locus maps to 7p12→cen. The human DNA complement of the hybrids was confirmed by Southern blot analysis using a large panel of chromosome 7-specific DNA probes. Several of these genes could be further sublocalized. These included: ARAF2 to 7pl2→cen. D7S21 to 7pter→p12, ACTB to 7p15→p12, EGFR to 7p12. MDH2 to 7cen→q22, and PDGFA to 7pter→p15.

Original languageEnglish
Pages (from-to)200-205
Number of pages6
JournalCytogenetic and Genome Research
Volume60
Issue number3-4
DOIs
Publication statusPublished - 1992
Externally publishedYes

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