Abstract
Phytophthora infestans is a devastating plant pathogen that causes late blight on potato and tomato. To colonize
host plants, P. infestans secretes effectors that can modulate host defence. Wellknown are the RXLR effectors,
which are able to translocate into host cells to manipulate the cell machinery. However, to counteract the pathogen potato has a set of immune receptors known as nucleotide-binding leucine-rich repeat (NLR) proteins
that confer resistance against P. infestans. NLR-conferred resistance is mediated by recognition of RXLR effectors, with each NLR protein (or R protein) having its own cognate RXLR effector (or AVR protein). The
mechanisms underlying NLR-mediated resistance are still poorly understood. In this study we focussed on the
P. infestans RXLR effector AVR1 and its cognate potato NLR R1 and addressed the question in which subcellular
compartment effector perception and defence activation takes place. We determined the subcellular localization
of both AVR1 and R1. We also fused Nuclear Localization Signals (NLS) and Nuclear Export Signals (NES) to R1
and AVR1, as well as mutated NLS and NES, and used these constructs for artificial subcellular targeting of R1
and AVR1. This allowed us to determine the subcellular localization that is required to elicit R1-mediated
immunity and AVR1-mediated host defence suppression
Original language | English |
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Title of host publication | Book of Abstracts Oomycete Molecular Genetics Network Meeting |
Pages | 11 |
Publication status | Published - 2014 |
Event | 2014 OMGN Meeting, Norwich, UK - Duration: 2 Jul 2014 → 4 Jul 2014 |
Conference
Conference | 2014 OMGN Meeting, Norwich, UK |
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Period | 2/07/14 → 4/07/14 |