Subcellular localization and kinase activity of GK4, a Phytophthora infestans GPCR-PIPK involved in actin cytoskeleton organisation

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

For dispersal and host infection plant pathogens largely depend on asexual spores. Pathogenesis and sporulation are complex processes that are governed by various cellular signaling networks including G-protein and phospholipid signaling. Oomycetes possess a family of novel proteins called GPCR-PIPKs (GKs) that are composed of a seven transmembrane spanning (7-TM) domain fused to a phosphatidylinositol phosphate kinase (PIPK) domain. Based on this domain structure GKs are anticipated to link G-protein and phospholipid signalling pathways. Our studies in the potato late blight pathogen Phytophthora infestans revealed involvement of one of twelve GKs (i.e. PiGK4) in spore development, hyphal elongation and infection. Moreover, ectopic expression in P. infestans of subdomains of PiGK1 and PiGK4 fused to a fluorescent protein showed that the GPCR domain targets the GKs to membranes surrounding different cellular compartments. To further elucidate the function of the PIPK domain we tested kinase activity of PiGK4 both in vivo and in vitro and analysed the relationship between PiGK4, phosphoinositide signaling and the organisation of the actin cytoskeleton using complementation in yeast combined with various live-cell markers.
Original languageEnglish
Title of host publicationBook of Abstracts Oomycete Molecular Genetics Network Meeting, Pacific Grove, California, USA, 10-12 March 2013
Pages43
Publication statusPublished - 2013
EventOomycete Molecular Genetics Network Meeting 2013 - Blacksburg, United States
Duration: 10 Mar 201312 Mar 2013

Conference

ConferenceOomycete Molecular Genetics Network Meeting 2013
CountryUnited States
CityBlacksburg
Period10/03/1312/03/13

Fingerprint

Phytophthora infestans
Phosphatidylinositol Phosphates
Actin Cytoskeleton
Phosphotransferases
Spores
GTP-Binding Proteins
Phospholipids
Oomycetes
Solanum tuberosum
Phosphatidylinositols
Infection
Proteins
Yeasts
Membranes

Cite this

Hua, C., Meijer, H. J. G., Kots, K., Ketelaar, T., & Govers, F. (2013). Subcellular localization and kinase activity of GK4, a Phytophthora infestans GPCR-PIPK involved in actin cytoskeleton organisation. In Book of Abstracts Oomycete Molecular Genetics Network Meeting, Pacific Grove, California, USA, 10-12 March 2013 (pp. 43)
Hua, C. ; Meijer, H.J.G. ; Kots, K. ; Ketelaar, T. ; Govers, F. / Subcellular localization and kinase activity of GK4, a Phytophthora infestans GPCR-PIPK involved in actin cytoskeleton organisation. Book of Abstracts Oomycete Molecular Genetics Network Meeting, Pacific Grove, California, USA, 10-12 March 2013. 2013. pp. 43
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abstract = "For dispersal and host infection plant pathogens largely depend on asexual spores. Pathogenesis and sporulation are complex processes that are governed by various cellular signaling networks including G-protein and phospholipid signaling. Oomycetes possess a family of novel proteins called GPCR-PIPKs (GKs) that are composed of a seven transmembrane spanning (7-TM) domain fused to a phosphatidylinositol phosphate kinase (PIPK) domain. Based on this domain structure GKs are anticipated to link G-protein and phospholipid signalling pathways. Our studies in the potato late blight pathogen Phytophthora infestans revealed involvement of one of twelve GKs (i.e. PiGK4) in spore development, hyphal elongation and infection. Moreover, ectopic expression in P. infestans of subdomains of PiGK1 and PiGK4 fused to a fluorescent protein showed that the GPCR domain targets the GKs to membranes surrounding different cellular compartments. To further elucidate the function of the PIPK domain we tested kinase activity of PiGK4 both in vivo and in vitro and analysed the relationship between PiGK4, phosphoinositide signaling and the organisation of the actin cytoskeleton using complementation in yeast combined with various live-cell markers.",
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Hua, C, Meijer, HJG, Kots, K, Ketelaar, T & Govers, F 2013, Subcellular localization and kinase activity of GK4, a Phytophthora infestans GPCR-PIPK involved in actin cytoskeleton organisation. in Book of Abstracts Oomycete Molecular Genetics Network Meeting, Pacific Grove, California, USA, 10-12 March 2013. pp. 43, Oomycete Molecular Genetics Network Meeting 2013, Blacksburg, United States, 10/03/13.

Subcellular localization and kinase activity of GK4, a Phytophthora infestans GPCR-PIPK involved in actin cytoskeleton organisation. / Hua, C.; Meijer, H.J.G.; Kots, K.; Ketelaar, T.; Govers, F.

Book of Abstracts Oomycete Molecular Genetics Network Meeting, Pacific Grove, California, USA, 10-12 March 2013. 2013. p. 43.

Research output: Chapter in Book/Report/Conference proceedingAbstract

TY - CHAP

T1 - Subcellular localization and kinase activity of GK4, a Phytophthora infestans GPCR-PIPK involved in actin cytoskeleton organisation

AU - Hua, C.

AU - Meijer, H.J.G.

AU - Kots, K.

AU - Ketelaar, T.

AU - Govers, F.

PY - 2013

Y1 - 2013

N2 - For dispersal and host infection plant pathogens largely depend on asexual spores. Pathogenesis and sporulation are complex processes that are governed by various cellular signaling networks including G-protein and phospholipid signaling. Oomycetes possess a family of novel proteins called GPCR-PIPKs (GKs) that are composed of a seven transmembrane spanning (7-TM) domain fused to a phosphatidylinositol phosphate kinase (PIPK) domain. Based on this domain structure GKs are anticipated to link G-protein and phospholipid signalling pathways. Our studies in the potato late blight pathogen Phytophthora infestans revealed involvement of one of twelve GKs (i.e. PiGK4) in spore development, hyphal elongation and infection. Moreover, ectopic expression in P. infestans of subdomains of PiGK1 and PiGK4 fused to a fluorescent protein showed that the GPCR domain targets the GKs to membranes surrounding different cellular compartments. To further elucidate the function of the PIPK domain we tested kinase activity of PiGK4 both in vivo and in vitro and analysed the relationship between PiGK4, phosphoinositide signaling and the organisation of the actin cytoskeleton using complementation in yeast combined with various live-cell markers.

AB - For dispersal and host infection plant pathogens largely depend on asexual spores. Pathogenesis and sporulation are complex processes that are governed by various cellular signaling networks including G-protein and phospholipid signaling. Oomycetes possess a family of novel proteins called GPCR-PIPKs (GKs) that are composed of a seven transmembrane spanning (7-TM) domain fused to a phosphatidylinositol phosphate kinase (PIPK) domain. Based on this domain structure GKs are anticipated to link G-protein and phospholipid signalling pathways. Our studies in the potato late blight pathogen Phytophthora infestans revealed involvement of one of twelve GKs (i.e. PiGK4) in spore development, hyphal elongation and infection. Moreover, ectopic expression in P. infestans of subdomains of PiGK1 and PiGK4 fused to a fluorescent protein showed that the GPCR domain targets the GKs to membranes surrounding different cellular compartments. To further elucidate the function of the PIPK domain we tested kinase activity of PiGK4 both in vivo and in vitro and analysed the relationship between PiGK4, phosphoinositide signaling and the organisation of the actin cytoskeleton using complementation in yeast combined with various live-cell markers.

M3 - Abstract

SP - 43

BT - Book of Abstracts Oomycete Molecular Genetics Network Meeting, Pacific Grove, California, USA, 10-12 March 2013

ER -

Hua C, Meijer HJG, Kots K, Ketelaar T, Govers F. Subcellular localization and kinase activity of GK4, a Phytophthora infestans GPCR-PIPK involved in actin cytoskeleton organisation. In Book of Abstracts Oomycete Molecular Genetics Network Meeting, Pacific Grove, California, USA, 10-12 March 2013. 2013. p. 43