Subcellular differences in post-translational modification of barley 14-3-3 proteins

Mieke J. Van Zeijl, Christa Testerink, Jan W. Kijne, Mei Wang*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

Expression and post-translational modification of barley 14-3-3 isoforms, 14-3-3A, 14-3-3B and 14-3-3C, were investigated using isoform-specific antibodies. Although all three isoforms were shown to be present in the cytosolic, the nuclear and the microsomal cell fractions, differences in post-translational modification were identified for the different cell fractions. Germination-related modifications of 14-3-3 proteins were observed in the cytosol and the microsomal fraction, but not in the nucleus. In vitro proteolytic cleavage of 14-3-3 proteins using trypsin suggests that for 14-3-3A this change was caused by proteolytic cleavage of the unconserved C-terminal region. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)292-296
Number of pages5
JournalFEBS Letters
Volume473
Issue number3
DOIs
Publication statusPublished - 19 May 2000
Externally publishedYes

Keywords

  • 14-3-3 Protein
  • Cell compartment
  • Germination
  • Hordeum distichum
  • Post-translational modification

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