Study of mycotoxin calibration approaches on the example of trichothecenes analysis from flour

Josep Rubert*, Kevin J. James, Jordi Mañes, Carla Soler

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)


The aim of this study was to evaluate the applicability of different calibration approaches in trichothecenes analysis from wheat flour. Concretely eight trichothecenes (five type B trichothecenes and three type A trichothecenes) were analyzed by matrix solid-phase dispersion (MSPD) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). In the first set of experiments the presence of matrix effects was evaluated; values ranged between 59% and 79%. In the second set of experiments, solutions to compensate these signal suppressions were examined. Different calibration methods showed to tackle matrix effects obtaining values between 69% and 85% for external matrix matched calibration and values from 76% to 111% and from 78% to 108% by analog and deuterated internal standard calibrations, respectively. Trueness of the method was studied using quality control material and certificated reference material (DON 1062±110μgkg -1). The values were compared by matrix-matched, analog internal standard (deepoxydeoxynivalenol (DOM-1)) and deuterated internal standard (DON-d 1).

Original languageEnglish
Pages (from-to)2034-2041
Number of pages8
JournalFood and Chemical Toxicology
Issue number6
Publication statusPublished - Jun 2012
Externally publishedYes


  • External matrix-matched calibration
  • Internal standard calibration
  • Mass spectrometry
  • Mycotoxins


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