Studies on the colonization of axenically grown tomato plants by a GFP-tagged strain of Clavibacter michiganensis subsp. michiganensis

F.M. Vieira Lelis, R.L. Czajkowski, R.M. de Souza, D.H. Ribeiro, J.M. van der Wolf

    Research output: Contribution to journalArticleAcademicpeer-review

    7 Citations (Scopus)

    Abstract

    In this study, colonization and disease development of axenically-grown tomato plants by Clavibacter michiganensis subsp. michiganensis (Cmm), the causative agent of bacterial wilt and canker, was investigated. For this, a spontaneous rifampicin resistant strain of Cmm was tagged with a marker that expressed a green fluorescent protein (GFP) in a stable way and which possessed a similar virulence to the parental strain. In vitro plants were drop-inoculated at the stem base and the population dynamics was determined by dilution pour-plating in a selective medium. At 3 h after inoculation, Cmm was already present in low densities in roots, stems and leaves. At 16 dpi, Cmm was found throughout the entire plant in high densities of ca. 1010 cfu g-1. Symptoms developed in the in vitro plants typical for Cmm, such as canker, wilting and growth reduction. The presence of Cmm in vascular and parenchymatic tissue of in vitro tomato plants was confirmed by epifluorescence stereo- and confocal laser scanning microscopy. This study showed that in vitro tomato plants can be effectively used for detailed studies on interactions between Cmm and its host, in particular if a GFP-tagged strain of the pathogen is used
    Original languageEnglish
    Pages (from-to)53-66
    JournalEuropean Journal of Plant Pathology
    Volume139
    Issue number1
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    Clavibacter michiganensis subsp. michiganensis
    green fluorescent protein
    tomatoes
    bacterial wilt
    rifampicin
    stems
    selective media
    confocal laser scanning microscopy
    wilting
    blood vessels
    in vitro studies
    growth retardation
    signs and symptoms (plants)
    virulence
    population dynamics
    pathogens

    Keywords

    • corynebacterium-michiganense
    • phytopathogenic bacterium
    • xylem sap
    • virulence
    • pathogens
    • tissue
    • seed
    • transmission
    • transplants
    • resistance

    Cite this

    @article{15301ef732504931986a39bbde89a66e,
    title = "Studies on the colonization of axenically grown tomato plants by a GFP-tagged strain of Clavibacter michiganensis subsp. michiganensis",
    abstract = "In this study, colonization and disease development of axenically-grown tomato plants by Clavibacter michiganensis subsp. michiganensis (Cmm), the causative agent of bacterial wilt and canker, was investigated. For this, a spontaneous rifampicin resistant strain of Cmm was tagged with a marker that expressed a green fluorescent protein (GFP) in a stable way and which possessed a similar virulence to the parental strain. In vitro plants were drop-inoculated at the stem base and the population dynamics was determined by dilution pour-plating in a selective medium. At 3 h after inoculation, Cmm was already present in low densities in roots, stems and leaves. At 16 dpi, Cmm was found throughout the entire plant in high densities of ca. 1010 cfu g-1. Symptoms developed in the in vitro plants typical for Cmm, such as canker, wilting and growth reduction. The presence of Cmm in vascular and parenchymatic tissue of in vitro tomato plants was confirmed by epifluorescence stereo- and confocal laser scanning microscopy. This study showed that in vitro tomato plants can be effectively used for detailed studies on interactions between Cmm and its host, in particular if a GFP-tagged strain of the pathogen is used",
    keywords = "corynebacterium-michiganense, phytopathogenic bacterium, xylem sap, virulence, pathogens, tissue, seed, transmission, transplants, resistance",
    author = "{Vieira Lelis}, F.M. and R.L. Czajkowski and {de Souza}, R.M. and D.H. Ribeiro and {van der Wolf}, J.M.",
    year = "2014",
    doi = "10.1007/s10658-013-0362-9",
    language = "English",
    volume = "139",
    pages = "53--66",
    journal = "European Journal of Plant Pathology",
    issn = "0929-1873",
    publisher = "Springer Verlag",
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    }

    Studies on the colonization of axenically grown tomato plants by a GFP-tagged strain of Clavibacter michiganensis subsp. michiganensis. / Vieira Lelis, F.M.; Czajkowski, R.L.; de Souza, R.M.; Ribeiro, D.H.; van der Wolf, J.M.

    In: European Journal of Plant Pathology, Vol. 139, No. 1, 2014, p. 53-66.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Studies on the colonization of axenically grown tomato plants by a GFP-tagged strain of Clavibacter michiganensis subsp. michiganensis

    AU - Vieira Lelis, F.M.

    AU - Czajkowski, R.L.

    AU - de Souza, R.M.

    AU - Ribeiro, D.H.

    AU - van der Wolf, J.M.

    PY - 2014

    Y1 - 2014

    N2 - In this study, colonization and disease development of axenically-grown tomato plants by Clavibacter michiganensis subsp. michiganensis (Cmm), the causative agent of bacterial wilt and canker, was investigated. For this, a spontaneous rifampicin resistant strain of Cmm was tagged with a marker that expressed a green fluorescent protein (GFP) in a stable way and which possessed a similar virulence to the parental strain. In vitro plants were drop-inoculated at the stem base and the population dynamics was determined by dilution pour-plating in a selective medium. At 3 h after inoculation, Cmm was already present in low densities in roots, stems and leaves. At 16 dpi, Cmm was found throughout the entire plant in high densities of ca. 1010 cfu g-1. Symptoms developed in the in vitro plants typical for Cmm, such as canker, wilting and growth reduction. The presence of Cmm in vascular and parenchymatic tissue of in vitro tomato plants was confirmed by epifluorescence stereo- and confocal laser scanning microscopy. This study showed that in vitro tomato plants can be effectively used for detailed studies on interactions between Cmm and its host, in particular if a GFP-tagged strain of the pathogen is used

    AB - In this study, colonization and disease development of axenically-grown tomato plants by Clavibacter michiganensis subsp. michiganensis (Cmm), the causative agent of bacterial wilt and canker, was investigated. For this, a spontaneous rifampicin resistant strain of Cmm was tagged with a marker that expressed a green fluorescent protein (GFP) in a stable way and which possessed a similar virulence to the parental strain. In vitro plants were drop-inoculated at the stem base and the population dynamics was determined by dilution pour-plating in a selective medium. At 3 h after inoculation, Cmm was already present in low densities in roots, stems and leaves. At 16 dpi, Cmm was found throughout the entire plant in high densities of ca. 1010 cfu g-1. Symptoms developed in the in vitro plants typical for Cmm, such as canker, wilting and growth reduction. The presence of Cmm in vascular and parenchymatic tissue of in vitro tomato plants was confirmed by epifluorescence stereo- and confocal laser scanning microscopy. This study showed that in vitro tomato plants can be effectively used for detailed studies on interactions between Cmm and its host, in particular if a GFP-tagged strain of the pathogen is used

    KW - corynebacterium-michiganense

    KW - phytopathogenic bacterium

    KW - xylem sap

    KW - virulence

    KW - pathogens

    KW - tissue

    KW - seed

    KW - transmission

    KW - transplants

    KW - resistance

    U2 - 10.1007/s10658-013-0362-9

    DO - 10.1007/s10658-013-0362-9

    M3 - Article

    VL - 139

    SP - 53

    EP - 66

    JO - European Journal of Plant Pathology

    JF - European Journal of Plant Pathology

    SN - 0929-1873

    IS - 1

    ER -