Studies on genetic and on chemically induced resistance of cucumber tissues to Cladosporium cucumerinum (Ell. & Arth.)

M.K.E.D. Fouad

Research output: Thesisinternal PhD, WU

Abstract

A detailed anatomical study was made of the host-parasite relations of cucumber seedlings inoculated with Cladosporium cucumerinum and of the effect of treatment with G33, the roots being immersed in a 100 p.p.m. solution of the compound for 48 h before inoculation. It was concluded that the resistance conferred by G33 was situated both in the cuticle and in the deeper tissues, and that the substance was effective only in cells appearing microscopically sound. The effect did not extend beyond the cuticle, and resistance did not seem to be due to plasmatic interaction.

Uptake by the roots of cucumber seedlings of 2,4-dinitrophenol, sodium diethyldithiocarbamate and sodium azide had no effect on either genetic resistance or that induced by G33. However narcosis of seedlings by exposure over solutions of 1. 5 % ether or 0.24 % chloroform caused breakdown of genetic resistance in the varieties Mabro and Proso, though these still remained resistant to Penicillium italicum and Botrytis cinerea (neither of which normally infects cucumber). But narcosis had no effect on the resistance to C. cucumerinum conferred by G33, which, unlike genetic resistance, seemed therefore unrelated to host metabolism. The sap expressed from resistant varieties and from the uninfected portions of older plants of a susceptible variety (Lange Gele Tros) did not inhibit growth of the pathogen in culture.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Oort, A.J.P., Promotor
Award date5 Jul 1956
Place of PublicationWageningen
Publisher
Publication statusPublished - 1956

Fingerprint

Cladosporium cucumerinum
induced resistance
genetic resistance
cucumbers
seedlings
Penicillium italicum
2,4-dinitrophenol
sodium azide
Botrytis cinerea
chloroform
sap
ethers
sodium
uptake mechanisms
parasites
metabolism
pathogens
tissues
cells

Keywords

  • plant pathogenic fungi
  • cucumis sativus
  • cucumbers

Cite this

@phdthesis{cc1735b9c9b94432bb1d89a65768bef8,
title = "Studies on genetic and on chemically induced resistance of cucumber tissues to Cladosporium cucumerinum (Ell. & Arth.)",
abstract = "A detailed anatomical study was made of the host-parasite relations of cucumber seedlings inoculated with Cladosporium cucumerinum and of the effect of treatment with G33, the roots being immersed in a 100 p.p.m. solution of the compound for 48 h before inoculation. It was concluded that the resistance conferred by G33 was situated both in the cuticle and in the deeper tissues, and that the substance was effective only in cells appearing microscopically sound. The effect did not extend beyond the cuticle, and resistance did not seem to be due to plasmatic interaction.Uptake by the roots of cucumber seedlings of 2,4-dinitrophenol, sodium diethyldithiocarbamate and sodium azide had no effect on either genetic resistance or that induced by G33. However narcosis of seedlings by exposure over solutions of 1. 5 {\%} ether or 0.24 {\%} chloroform caused breakdown of genetic resistance in the varieties Mabro and Proso, though these still remained resistant to Penicillium italicum and Botrytis cinerea (neither of which normally infects cucumber). But narcosis had no effect on the resistance to C. cucumerinum conferred by G33, which, unlike genetic resistance, seemed therefore unrelated to host metabolism. The sap expressed from resistant varieties and from the uninfected portions of older plants of a susceptible variety (Lange Gele Tros) did not inhibit growth of the pathogen in culture.",
keywords = "plantenziekteverwekkende schimmels, cucumis sativus, komkommers, plant pathogenic fungi, cucumis sativus, cucumbers",
author = "M.K.E.D. Fouad",
note = "WU thesis 211 Proefschrift Wageningen",
year = "1956",
language = "English",
publisher = "Veenman",
school = "Wageningen University",

}

Studies on genetic and on chemically induced resistance of cucumber tissues to Cladosporium cucumerinum (Ell. & Arth.). / Fouad, M.K.E.D.

Wageningen : Veenman, 1956. 56 p.

Research output: Thesisinternal PhD, WU

TY - THES

T1 - Studies on genetic and on chemically induced resistance of cucumber tissues to Cladosporium cucumerinum (Ell. & Arth.)

AU - Fouad, M.K.E.D.

N1 - WU thesis 211 Proefschrift Wageningen

PY - 1956

Y1 - 1956

N2 - A detailed anatomical study was made of the host-parasite relations of cucumber seedlings inoculated with Cladosporium cucumerinum and of the effect of treatment with G33, the roots being immersed in a 100 p.p.m. solution of the compound for 48 h before inoculation. It was concluded that the resistance conferred by G33 was situated both in the cuticle and in the deeper tissues, and that the substance was effective only in cells appearing microscopically sound. The effect did not extend beyond the cuticle, and resistance did not seem to be due to plasmatic interaction.Uptake by the roots of cucumber seedlings of 2,4-dinitrophenol, sodium diethyldithiocarbamate and sodium azide had no effect on either genetic resistance or that induced by G33. However narcosis of seedlings by exposure over solutions of 1. 5 % ether or 0.24 % chloroform caused breakdown of genetic resistance in the varieties Mabro and Proso, though these still remained resistant to Penicillium italicum and Botrytis cinerea (neither of which normally infects cucumber). But narcosis had no effect on the resistance to C. cucumerinum conferred by G33, which, unlike genetic resistance, seemed therefore unrelated to host metabolism. The sap expressed from resistant varieties and from the uninfected portions of older plants of a susceptible variety (Lange Gele Tros) did not inhibit growth of the pathogen in culture.

AB - A detailed anatomical study was made of the host-parasite relations of cucumber seedlings inoculated with Cladosporium cucumerinum and of the effect of treatment with G33, the roots being immersed in a 100 p.p.m. solution of the compound for 48 h before inoculation. It was concluded that the resistance conferred by G33 was situated both in the cuticle and in the deeper tissues, and that the substance was effective only in cells appearing microscopically sound. The effect did not extend beyond the cuticle, and resistance did not seem to be due to plasmatic interaction.Uptake by the roots of cucumber seedlings of 2,4-dinitrophenol, sodium diethyldithiocarbamate and sodium azide had no effect on either genetic resistance or that induced by G33. However narcosis of seedlings by exposure over solutions of 1. 5 % ether or 0.24 % chloroform caused breakdown of genetic resistance in the varieties Mabro and Proso, though these still remained resistant to Penicillium italicum and Botrytis cinerea (neither of which normally infects cucumber). But narcosis had no effect on the resistance to C. cucumerinum conferred by G33, which, unlike genetic resistance, seemed therefore unrelated to host metabolism. The sap expressed from resistant varieties and from the uninfected portions of older plants of a susceptible variety (Lange Gele Tros) did not inhibit growth of the pathogen in culture.

KW - plantenziekteverwekkende schimmels

KW - cucumis sativus

KW - komkommers

KW - plant pathogenic fungi

KW - cucumis sativus

KW - cucumbers

M3 - internal PhD, WU

PB - Veenman

CY - Wageningen

ER -