Studies of the silencing of Baculovirus DNA binding protein

I. Quadt, J.W.M. van Lent, D. Knebel-Morsdorf

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

Baculovirus DNA binding protein (DBP) binds preferentially single-stranded DNA in vitro and colocalizes with viral DNA replication sites. Here, its putative role as viral replication factor has been addressed by RNA interference. Silencing of DBP in Autographa californica multiple nucleopolyhedrovirus-infected cells increased expression of LEF-3, LEF-4, and P35. In contrast, expression of the structural genes coding for P39 and polyhedrin was suppressed while expression of genes coding for P10 and GP64 was unaffected. In the absence of DBP, viral DNA replication sites were formed, indicating replication of viral DNA. Electron microscopy studies, however, revealed a loss of formation of polyhedra and virus envelopment, suggesting that the primary role of DBP is viral formation rather than viral DNA replication.
Original languageEnglish
Pages (from-to)6122-6127
JournalJournal of Virology
Volume81
Issue number11
DOIs
Publication statusPublished - 2007

Keywords

  • nuclear polyhedrosis-virus
  • replication sites
  • rna interference
  • infected-cells
  • in-vitro
  • lef-3
  • gene
  • expression
  • apoptosis
  • ie2

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