Structure of the lipodepsipeptide syringomycin e in phospholipids and sodium dodecylsulphate micelle studied by circular dichroism, NMR spectroscopy and molecular dynamics

Massimiliano Anselmi, Tommaso Eliseo, Laura Zanetti-Polzi, Maria Rosaria Fullone, Vincenzo Fogliano, Alfredo Di Nola, Maurizio Paci, Ingeborg Grgurina*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

Syringomycin E (SRE) is a member of a family of lipodepsipeptides that characterize the secondary metabolism of the plant-associated bacteria Pseudomonas syringae pv. syringae. It displays phytotoxic, antifungal and haemolytic activities, due to the membrane interaction and ion channel formation. To gain an insight into the conformation of SRE in the membrane environment, we studied the conformation of SRE bound to SDS micelle, a suitable model for the membrane-bound SRE. In fact, highly similar circular dichroism (CD) spectra were obtained for SRE bound to sodium dodecylsulphate (SDS) and to a phospholipid bilayer, indicating the conformational equivalence of SRE in these two media, at difference with the CD spectrum of SRE in water solution. The structure of SDS-bound SRE was determined by NMR spectroscopy combined with molecular dynamics calculations in octane environment. The results of this study highlight the influence of the interaction with lipids in determining the three-dimensional structure of SRE and provide the basis for further investigations on structural determinants of syringomycin E-membrane interaction.

Original languageEnglish
Pages (from-to)2102-2110
Number of pages9
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1808
Issue number9
DOIs
Publication statusPublished - Sep 2011
Externally publishedYes

Keywords

  • Antifungal lipodepsipeptide
  • Molecular dynamics
  • NMR spectroscopy
  • Phospholipid
  • SDS micelle
  • Syringomycin E conformation

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