Structural study of a pectic polysaccharide fraction isolated from “mountain tea” (Sideritis scardica Griseb.)

Manol Ognyanov*, Connie A. Remoroza, Henk A. Schols, Nadezhda Tr Petkova, Yordan N. Georgiev

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The present study was conducted to investigate the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea. The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%). The fraction contained mostly a highly methyl-esterified homogalacturonan (HG) – 71 mol%. The pectin had a high molecular weight population (∼60−100 kDa). Enzymatic fingerprinting was employed with a combination of HG degrading enzymes and LC-HILIC-MS, HPAEC, HPSEC to examine the structure in greater detail. Unsaturated oligomers released indicated the presence of large blocks of highly methyl-esterified GalA residues. Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated. The research findings provide a basis for further investigations regarding biological activity and commercial exploitation of mountain tea.

Original languageEnglish
Article number117798
JournalCarbohydrate Polymers
Volume260
DOIs
Publication statusPublished - 15 May 2021

Keywords

  • Enzymatic fingerprinting
  • HILIC-MS
  • Homogalacturonan
  • Mountain tea
  • Pectin
  • Sideritis sp.

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