Structural study of a pectic polysaccharide fraction isolated from “mountain tea” (Sideritis scardica Griseb.)

Manol Ognyanov; Connie A. Remoroza; Henk A. Schols; Nadezhda Tr Petkova; Yordan N. Georgiev

doi:10.1016/j.carbpol.2021.117798

Structural study of a pectic polysaccharide fraction isolated from “mountain tea” (Sideritis scardica Griseb.)

Manol Ognyanov^*, Connie A. Remoroza, Henk A. Schols, Nadezhda Tr Petkova, Yordan N. Georgiev

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

22 Citations (Scopus)

Abstract

The present study was conducted to investigate the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea. The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%). The fraction contained mostly a highly methyl-esterified homogalacturonan (HG) – 71 mol%. The pectin had a high molecular weight population (∼60−100 kDa). Enzymatic fingerprinting was employed with a combination of HG degrading enzymes and LC-HILIC-MS, HPAEC, HPSEC to examine the structure in greater detail. Unsaturated oligomers released indicated the presence of large blocks of highly methyl-esterified GalA residues. Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated. The research findings provide a basis for further investigations regarding biological activity and commercial exploitation of mountain tea.

Original language	English
Article number	117798
Journal	Carbohydrate Polymers
Volume	260
DOIs	https://doi.org/10.1016/j.carbpol.2021.117798
Publication status	Published - 15 May 2021

Keywords

Enzymatic fingerprinting
HILIC-MS
Homogalacturonan
Mountain tea
Pectin
Sideritis sp.

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10.1016/j.carbpol.2021.117798

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@article{7104e84065e6491e91d88f3d76994a48,

title = "Structural study of a pectic polysaccharide fraction isolated from “mountain tea” (Sideritis scardica Griseb.)",

abstract = "The present study was conducted to investigate the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea. The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%). The fraction contained mostly a highly methyl-esterified homogalacturonan (HG) – 71 mol%. The pectin had a high molecular weight population (∼60−100 kDa). Enzymatic fingerprinting was employed with a combination of HG degrading enzymes and LC-HILIC-MS, HPAEC, HPSEC to examine the structure in greater detail. Unsaturated oligomers released indicated the presence of large blocks of highly methyl-esterified GalA residues. Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated. The research findings provide a basis for further investigations regarding biological activity and commercial exploitation of mountain tea.",

keywords = "Enzymatic fingerprinting, HILIC-MS, Homogalacturonan, Mountain tea, Pectin, Sideritis sp.",

author = "Manol Ognyanov and Remoroza, {Connie A.} and Schols, {Henk A.} and Petkova, {Nadezhda Tr} and Georgiev, {Yordan N.}",

year = "2021",

month = may,

day = "15",

doi = "10.1016/j.carbpol.2021.117798",

language = "English",

volume = "260",

journal = "Carbohydrate Polymers",

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T1 - Structural study of a pectic polysaccharide fraction isolated from “mountain tea” (Sideritis scardica Griseb.)

AU - Ognyanov, Manol

AU - Remoroza, Connie A.

AU - Schols, Henk A.

AU - Petkova, Nadezhda Tr

AU - Georgiev, Yordan N.

PY - 2021/5/15

Y1 - 2021/5/15

N2 - The present study was conducted to investigate the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea. The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%). The fraction contained mostly a highly methyl-esterified homogalacturonan (HG) – 71 mol%. The pectin had a high molecular weight population (∼60−100 kDa). Enzymatic fingerprinting was employed with a combination of HG degrading enzymes and LC-HILIC-MS, HPAEC, HPSEC to examine the structure in greater detail. Unsaturated oligomers released indicated the presence of large blocks of highly methyl-esterified GalA residues. Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated. The research findings provide a basis for further investigations regarding biological activity and commercial exploitation of mountain tea.

AB - The present study was conducted to investigate the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea. The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%). The fraction contained mostly a highly methyl-esterified homogalacturonan (HG) – 71 mol%. The pectin had a high molecular weight population (∼60−100 kDa). Enzymatic fingerprinting was employed with a combination of HG degrading enzymes and LC-HILIC-MS, HPAEC, HPSEC to examine the structure in greater detail. Unsaturated oligomers released indicated the presence of large blocks of highly methyl-esterified GalA residues. Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated. The research findings provide a basis for further investigations regarding biological activity and commercial exploitation of mountain tea.

KW - Enzymatic fingerprinting

KW - HILIC-MS

KW - Homogalacturonan

KW - Mountain tea

KW - Pectin

KW - Sideritis sp.

U2 - 10.1016/j.carbpol.2021.117798

DO - 10.1016/j.carbpol.2021.117798

M3 - Article

AN - SCOPUS:85101332038

SN - 0144-8617

VL - 260

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

M1 - 117798

ER -

Structural study of a pectic polysaccharide fraction isolated from “mountain tea” (Sideritis scardica Griseb.)

Abstract

Keywords

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