Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek

Manol Ognyanov*, Connie Remoroza, Henk A. Schols, Yordan N. Georgiev, Nadezhda Tr Petkova, Magdalena Krystyjan

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

An acid-extracted polysaccharide from alchohol-insoluble solids of leek was obtained. The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose. The fraction contained a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains. The fraction consisted of three high Mw populations, covering the range of 10–100 kDa. Enzymatic fingerprinting was performed with HG/RG-I degrading enzymes to elucidate the structure. The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length. The rheological study showed the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide. The pectin exhibited higher water holding capacity than oil-holding capacity and the fraction did form stable foams at high concentration.

Original languageEnglish
Article number115549
JournalCarbohydrate Polymers
Volume229
DOIs
Publication statusPublished - 1 Feb 2020

Keywords

  • Enzymatic fingerprinting
  • HILIC-MS
  • Leek
  • Pectic polysaccharides
  • Rheology

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