Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek

Manol Ognyanov*, Connie Remoroza, Henk A. Schols, Yordan N. Georgiev, Nadezhda Tr Petkova, Magdalena Krystyjan

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

An acid-extracted polysaccharide from alchohol-insoluble solids of leek was obtained. The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose. The fraction contained a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains. The fraction consisted of three high Mw populations, covering the range of 10–100 kDa. Enzymatic fingerprinting was performed with HG/RG-I degrading enzymes to elucidate the structure. The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length. The rheological study showed the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide. The pectin exhibited higher water holding capacity than oil-holding capacity and the fraction did form stable foams at high concentration.

Original languageEnglish
Article number115549
JournalCarbohydrate Polymers
Volume229
DOIs
Publication statusPublished - 1 Feb 2020

Fingerprint

Polysaccharides
Galactose
Sugars
Galactans
Rhamnose
Arabinose
Shear thinning
Acids
Esterification
Oligomers
Foams
Oils
Enzymes
Water
Chemical analysis
rhamnogalacturonan I
pectin
polygalacturonic acid
galacturonic acid

Keywords

  • Enzymatic fingerprinting
  • HILIC-MS
  • Leek
  • Pectic polysaccharides
  • Rheology

Cite this

Ognyanov, Manol ; Remoroza, Connie ; Schols, Henk A. ; Georgiev, Yordan N. ; Petkova, Nadezhda Tr ; Krystyjan, Magdalena. / Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek. In: Carbohydrate Polymers. 2020 ; Vol. 229.
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abstract = "An acid-extracted polysaccharide from alchohol-insoluble solids of leek was obtained. The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose. The fraction contained a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains. The fraction consisted of three high Mw populations, covering the range of 10–100 kDa. Enzymatic fingerprinting was performed with HG/RG-I degrading enzymes to elucidate the structure. The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length. The rheological study showed the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide. The pectin exhibited higher water holding capacity than oil-holding capacity and the fraction did form stable foams at high concentration.",
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Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek. / Ognyanov, Manol; Remoroza, Connie; Schols, Henk A.; Georgiev, Yordan N.; Petkova, Nadezhda Tr; Krystyjan, Magdalena.

In: Carbohydrate Polymers, Vol. 229, 115549, 01.02.2020.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Structural, rheological and functional properties of galactose-rich pectic polysaccharide fraction from leek

AU - Ognyanov, Manol

AU - Remoroza, Connie

AU - Schols, Henk A.

AU - Georgiev, Yordan N.

AU - Petkova, Nadezhda Tr

AU - Krystyjan, Magdalena

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N2 - An acid-extracted polysaccharide from alchohol-insoluble solids of leek was obtained. The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose. The fraction contained a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains. The fraction consisted of three high Mw populations, covering the range of 10–100 kDa. Enzymatic fingerprinting was performed with HG/RG-I degrading enzymes to elucidate the structure. The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length. The rheological study showed the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide. The pectin exhibited higher water holding capacity than oil-holding capacity and the fraction did form stable foams at high concentration.

AB - An acid-extracted polysaccharide from alchohol-insoluble solids of leek was obtained. The sugar composition indicated that galactose and galacturonic acid were the major sugars, followed by small amounts of rhamnose and arabinose. The fraction contained a relatively high methyl-esterified homogalacturonan next to rhamnogalacturonan type I decorated with galactose-rich side chains. The fraction consisted of three high Mw populations, covering the range of 10–100 kDa. Enzymatic fingerprinting was performed with HG/RG-I degrading enzymes to elucidate the structure. The oligomers were analysed using LC-HILIC-MS, HPAEC, and MALDI-TOF MS. The data revealed the presence of GalA sequences, having different patterns of methyl-esterification, RG-I composed of unbranched segments and segments heavily substituted with β-(1→4)-linked galactan chains of varying length. The rheological study showed the shear-thinning, weak thixotropic, anti-thixotropic, and non-Newtonian behavior of the polysaccharide. The pectin exhibited higher water holding capacity than oil-holding capacity and the fraction did form stable foams at high concentration.

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