Structural heterogeneity in DNA : Temperature dependence of 2-aminopurine fluorescence in dinucleotides

O.J.G. Somsen, L. Keukens, M.N. de Keijzer, A. van Hoek, H. van Amerongen

Research output: Contribution to journalArticleAcademicpeer-review

35 Citations (Scopus)

Abstract

The fluorescent base analogue 2-aminopurine is a sensitive probe for local dynamics of DNA. Its fluorescence is quenched by interaction with the neighboring bases, but the underlying mechanisms are still under investigation. We studied 2-aminopurine fluorescence in dinucleotides with each of the natural bases. Consistently, two of the four fluorescence-decay components depend strongly on temperature. Our results indicate that these components are due to the excited-state dynamics of a single conformational state. We propose a variation of the gating model in which transient unstacking occurs in the excited state.
Original languageEnglish
Pages (from-to)1622-1627
Number of pages6
JournalChemPhysChem
Volume6
Issue number8
DOIs
Publication statusPublished - 2005

Keywords

  • ultrafast transient-absorption
  • time-resolved fluorescence
  • charge-transfer
  • electron-transfer
  • up-conversion
  • dynamics
  • base
  • nucleotides
  • rna
  • spectroscopy

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