Structural characterization of tissue-specific galactan from flax fibers by 1H NMR and MALDI TOF mass spectrosmetry

O.P. Gur'janov; T. Gorshkova; M.A. Kabel; H.A. Schols; J.E.G. van Dam

doi:10.1134/S1068162006060094

Structural characterization of tissue-specific galactan from flax fibers by 1H NMR and MALDI TOF mass spectrosmetry

O.P. Gur'janov, T. Gorshkova, M.A. Kabel, H.A. Schols, J.E.G. van Dam

Research output: Contribution to journal › Article › Academic › peer-review

5 Citations (Scopus)

Abstract

A high-molecular-mass polysaccharide galactan (M 2000 kDa) was isolated from flax at the stage of cell wall thickening of the bast fiber development. The polymer structure was studied by 1H NMR spectroscopy and MALDI TOF mass spectrometry. It is built up of Gal (59%), Rha (15%), GalA (23%), and Ara (3%) residues. The galactan backbone consists of successively alternating monomer disaccharide units (- 4GalA1 - 2Rha1 -)n and is similar in its structure to the backbone of rhamnogalacturonan-1 (RG-I). Rhamnose residues bear in position 4 ß-(1 - 4)-galactose side chains of various lengths with a polymerization degree of up to 28 or higher. A part of the side chains have branchings.

Original language	English
Pages (from-to)	558-567
Journal	Russian Journal of Bioorganic Chemistry
Volume	32
Issue number	6
DOIs	https://doi.org/10.1134/S1068162006060094
Publication status	Published - 2006

Keywords

plant-cell walls
linum-usitatissimum l
rhamnogalacturonan-i
polysaccharides
oligosaccharides
identification
spectroscopy
transition
pectins
complex

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10.1134/S1068162006060094

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title = "Structural characterization of tissue-specific galactan from flax fibers by 1H NMR and MALDI TOF mass spectrosmetry",

abstract = "A high-molecular-mass polysaccharide galactan (M 2000 kDa) was isolated from flax at the stage of cell wall thickening of the bast fiber development. The polymer structure was studied by 1H NMR spectroscopy and MALDI TOF mass spectrometry. It is built up of Gal (59%), Rha (15%), GalA (23%), and Ara (3%) residues. The galactan backbone consists of successively alternating monomer disaccharide units (- 4GalA1 - 2Rha1 -)n and is similar in its structure to the backbone of rhamnogalacturonan-1 (RG-I). Rhamnose residues bear in position 4 {\ss}-(1 - 4)-galactose side chains of various lengths with a polymerization degree of up to 28 or higher. A part of the side chains have branchings.",

keywords = "plant-cell walls, linum-usitatissimum l, rhamnogalacturonan-i, polysaccharides, oligosaccharides, identification, spectroscopy, transition, pectins, complex",

author = "O.P. Gur'janov and T. Gorshkova and M.A. Kabel and H.A. Schols and {van Dam}, J.E.G.",

year = "2006",

doi = "10.1134/S1068162006060094",

language = "English",

volume = "32",

pages = "558--567",

journal = "Russian Journal of Bioorganic Chemistry",

issn = "1068-1620",

publisher = "Pleiades Publishing",

number = "6",

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TY - JOUR

T1 - Structural characterization of tissue-specific galactan from flax fibers by 1H NMR and MALDI TOF mass spectrosmetry

AU - Gur'janov, O.P.

AU - Gorshkova, T.

AU - Kabel, M.A.

AU - Schols, H.A.

AU - van Dam, J.E.G.

PY - 2006

Y1 - 2006

N2 - A high-molecular-mass polysaccharide galactan (M 2000 kDa) was isolated from flax at the stage of cell wall thickening of the bast fiber development. The polymer structure was studied by 1H NMR spectroscopy and MALDI TOF mass spectrometry. It is built up of Gal (59%), Rha (15%), GalA (23%), and Ara (3%) residues. The galactan backbone consists of successively alternating monomer disaccharide units (- 4GalA1 - 2Rha1 -)n and is similar in its structure to the backbone of rhamnogalacturonan-1 (RG-I). Rhamnose residues bear in position 4 ß-(1 - 4)-galactose side chains of various lengths with a polymerization degree of up to 28 or higher. A part of the side chains have branchings.

AB - A high-molecular-mass polysaccharide galactan (M 2000 kDa) was isolated from flax at the stage of cell wall thickening of the bast fiber development. The polymer structure was studied by 1H NMR spectroscopy and MALDI TOF mass spectrometry. It is built up of Gal (59%), Rha (15%), GalA (23%), and Ara (3%) residues. The galactan backbone consists of successively alternating monomer disaccharide units (- 4GalA1 - 2Rha1 -)n and is similar in its structure to the backbone of rhamnogalacturonan-1 (RG-I). Rhamnose residues bear in position 4 ß-(1 - 4)-galactose side chains of various lengths with a polymerization degree of up to 28 or higher. A part of the side chains have branchings.

KW - plant-cell walls

KW - linum-usitatissimum l

KW - rhamnogalacturonan-i

KW - polysaccharides

KW - oligosaccharides

KW - identification

KW - spectroscopy

KW - transition

KW - pectins

KW - complex

U2 - 10.1134/S1068162006060094

DO - 10.1134/S1068162006060094

M3 - Article

SN - 1068-1620

VL - 32

SP - 558

EP - 567

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

IS - 6

ER -

Structural characterization of tissue-specific galactan from flax fibers by 1H NMR and MALDI TOF mass spectrosmetry

Abstract

Keywords

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