Structural basis for CRISPR RNA-guided DNA recognition by Cascade

M.M. Jore, N.M.J. Lundgren, E. van Duijn, J.B. Bultema, E.R. Westra, J. van der Oost, S.J.J. Brouns, M.R. Beijer

Research output: Contribution to journalArticleAcademicpeer-review

374 Citations (Scopus)

Abstract

The CRISPR (clustered regularly interspaced short palindromic repeats) immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids. In Escherichia coli, immunity depends on a ribonucleoprotein complex called Cascade. Here we present the composition and low-resolution structure of Cascade and show how it recognizes double-stranded DNA (dsDNA) targets in a sequence-specific manner. Cascade is a 405-kDa complex comprising five functionally essential CRISPR-associated (Cas) proteins (CasA1B2C6D1E1) and a 61-nucleotide CRISPR RNA (crRNA) with 5'-hydroxyl and 2',3'-cyclic phosphate termini. The crRNA guides Cascade to dsDNA target sequences by forming base pairs with the complementary DNA strand while displacing the noncomplementary strand to form an R-loop. Cascade recognizes target DNA without consuming ATP, which suggests that continuous invader DNA surveillance takes place without energy investment. The structure of Cascade shows an unusual seahorse shape that undergoes conformational changes when it binds target DNA
Original languageEnglish
Pages (from-to)529-536
JournalNature Structural and Molecular Biology
Volume18
Issue number5
DOIs
Publication statusPublished - 2011

Keywords

  • of-flight instrument
  • mass-spectrometry
  • r-loops
  • streptococcus-thermophilus
  • archaeoglobus-fulgidus
  • immune-system
  • protein
  • defense
  • prokaryotes
  • bacteria

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