Structural and functional analysis of Eukaryal-like proteins from the hyperthermophilic archaeon Sulfolobus solfataricus

Wu Hao

Research output: Thesisinternal PhD, WU

Abstract

The research presented in this thesis is aimed at applying technologies in bioinformatics, biochemistry, structural biology and cell biology to reveal the global regulation network in archaea, gain insights in the mechanism of archaeal signal transduction, and provide details on the evolution of the well-conserved archaeal-eukaryal information processing systems (i.e. transcription, translation, and replication). The global regulation network includes several novel core eukaryal-like proteins (e.g. MBF and SsGBP) that are predicted to operate in the regulation of transcription and/or translation in archaea. In addition, approaches are described to analyze the function of the predicted regulators that involved the development of  antibiotic resistance marker for hyperthermophiles since the genetic modification of hyperthermophiles has been hampered, at least in part, by the lack of suitable selection markers.      
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • van der Oost, John, Promotor
  • de Vos, Willem, Promotor
  • Rao, Z., Co-promotor, External person
Award date10 Dec 2007
Place of Publication[S.l.]
Print ISBNs9789085048220
Publication statusPublished - 2007

Keywords

  • proteins
  • molecular conformation
  • protein utilization
  • alpha-galactosidase
  • archaea

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