Strigolactone-triggered stomatal closure requires hydrogen peroxide synthesis and nitric oxide production in an abscisic acid-independent manner

Shuo Lv, Yonghong Zhang, Chen Yan Li, Zhijun Liu, Nan Yang, Lixia Pan, Jinbin Wu, Jiajing Wang, Jingwei Yang, Yanting Lv, Yutao Zhang, Wenqian Jiang, Xiaoping She, Guodong Wang*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

85 Citations (Scopus)

Abstract

Accumulating data indicate that strigolactones (SLs) are implicated in the response to environmental stress, implying a potential effect of SLs on stomatal response and thus stress acclimatization. In this study, we investigated the molecular mechanism underlying the effect of SLs on stomatal response and their interrelation with abscisic acid (ABA) signaling. The impact of SLs on the stomatal response was investigated by conducting SL-feeding experiments and by analyzing SL-related mutants. The involvement of endogenous ABA and ABA-signaling components in SL-mediated stomatal closure was physiologically evaluated using genetic mutants. Pharmacological and genetic approaches were employed to examine hydrogen peroxide (H2O2) and nitric oxide (NO) production. SL-related mutants exhibited larger stomatal apertures, while exogenous SLs were able to induce stomatal closure and rescue the more widely opening stomata of SL-deficient mutants. The SL-biosynthetic genes were induced by abiotic stress in shoot tissues. Disruption of ABA-biosynthetic genes, as well as genes that function in guard cell ABA signaling, resulted in no impairment in SL-mediated stomatal response. However, disruption of MORE AXILLARY GROWTH2 (MAX2), DWARF14 (D14), and the anion channel gene SLOW ANION CHANNEL-ASSOCIATED 1 (SLAC1) impaired SL-triggered stomatal closure. SLs stimulated a marked increase in H2O2 and NO contents, which is required for stomatal closure. Our results suggest that SLs play a prominent role, together with H2O2/NO production and SLAC1 activation, in inducing stomatal closure in an ABA-independent mechanism.

Original languageEnglish
Pages (from-to)290-304
JournalNew Phytologist
Volume217
Issue number1
Early online date22 Sep 2017
DOIs
Publication statusPublished - Jan 2018

Keywords

  • Abscisic acid (ABA)
  • Arabidopsis thaliana
  • Hydrogen peroxide (HO)
  • Nitric oxide (NO)
  • Stomata
  • Strigolactone

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