Streptococcus caviae sp. nov., isolated from Guinea pig faecal samples

Susakul Palakawong Na Ayudthaya*, Loes J. Hilderink, John van der Oost, Willem M. de Vos, Caroline M. Plugge

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

A novel cellobiose-degrading and lactate-producing bacterium, strain Cavy grass 6T, was isolated from faecal samples of guinea pigs (Cavia porcellus). Cells of the strain were ovalshaped, non-motile, non-spore-forming, Gram-stain-positive and facultatively anaerobic. The strain gr at 25–40 °C (optimum 37 °C) and pH 4.5–9.5 (optimum 8.0). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Cavy grass 6T belongs to the genus Streptococcus with its closest relative being Streptococcus devriesei CCUG 47155T with only 96.5% similarity. Comparing strain Cavy grass 6T and Streptococcus devriesei CCUG 47155T, average nucleotide identity and level of digital DNA–DNA hybridization dDDH were only 86.9 and 33.3%, respectively. Housekeeping genes groEL and gyrA were different between strain Cavy grass 6T and other streptococci. The G+C content of strain Cavy grass 6T was 42.6±0.3 mol%. The major (>10%) cellular fatty acids of strain Cavy grass 6T were C16:0, C20: 1ω9c and summed feature 8 (C18: 1ω7c and/or C18: 1ω6c). Strain Cavy grass 6T ferment a range of plant mono- and disaccharides as well as polymeric carbohydrates, including cellobiose, dulcitol, D-glucose, maltose, raffinose, sucrose, L-sorbose, trehalose, inulin and dried grass extract, to lactate, formate, acetate and ethanol. Based on phylogenetic and physiological characteristics, Cavy grass 6T can be distinguished from other members of the genus Streptococcus. Therefore, a novel species of the genus Streptococcus, family Streptococcaceae, order Lactobacillales is proposed, Streptococcus caviae sp. nov. (type strain Cavy grass 6T=TISTR 2371T=DSM 102819T).

Original languageEnglish
Pages (from-to)1551-1556
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number5
DOIs
Publication statusPublished - 2017

Keywords

  • Cellobiose
  • Dried grass
  • Fermentation
  • Inulin
  • Lactic acid
  • Streptococcus

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