TY - JOUR
T1 - Streptococcus caviae sp. nov., isolated from Guinea pig faecal samples
AU - Palakawong Na Ayudthaya, Susakul
AU - Hilderink, Loes J.
AU - van der Oost, John
AU - de Vos, Willem M.
AU - Plugge, Caroline M.
PY - 2017
Y1 - 2017
N2 - A novel cellobiose-degrading and lactate-producing bacterium, strain Cavy grass 6T, was isolated from faecal samples of guinea pigs (Cavia porcellus). Cells of the strain were ovalshaped, non-motile, non-spore-forming, Gram-stain-positive and facultatively anaerobic. The strain gr at 25–40 °C (optimum 37 °C) and pH 4.5–9.5 (optimum 8.0). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Cavy grass 6T belongs to the genus Streptococcus with its closest relative being Streptococcus devriesei CCUG 47155T with only 96.5% similarity. Comparing strain Cavy grass 6T and Streptococcus devriesei CCUG 47155T, average nucleotide identity and level of digital DNA–DNA hybridization dDDH were only 86.9 and 33.3%, respectively. Housekeeping genes groEL and gyrA were different between strain Cavy grass 6T and other streptococci. The G+C content of strain Cavy grass 6T was 42.6±0.3 mol%. The major (>10%) cellular fatty acids of strain Cavy grass 6T were C16:0, C20: 1ω9c and summed feature 8 (C18: 1ω7c and/or C18: 1ω6c). Strain Cavy grass 6T ferment a range of plant mono- and disaccharides as well as polymeric carbohydrates, including cellobiose, dulcitol, D-glucose, maltose, raffinose, sucrose, L-sorbose, trehalose, inulin and dried grass extract, to lactate, formate, acetate and ethanol. Based on phylogenetic and physiological characteristics, Cavy grass 6T can be distinguished from other members of the genus Streptococcus. Therefore, a novel species of the genus Streptococcus, family Streptococcaceae, order Lactobacillales is proposed, Streptococcus caviae sp. nov. (type strain Cavy grass 6T=TISTR 2371T=DSM 102819T).
AB - A novel cellobiose-degrading and lactate-producing bacterium, strain Cavy grass 6T, was isolated from faecal samples of guinea pigs (Cavia porcellus). Cells of the strain were ovalshaped, non-motile, non-spore-forming, Gram-stain-positive and facultatively anaerobic. The strain gr at 25–40 °C (optimum 37 °C) and pH 4.5–9.5 (optimum 8.0). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Cavy grass 6T belongs to the genus Streptococcus with its closest relative being Streptococcus devriesei CCUG 47155T with only 96.5% similarity. Comparing strain Cavy grass 6T and Streptococcus devriesei CCUG 47155T, average nucleotide identity and level of digital DNA–DNA hybridization dDDH were only 86.9 and 33.3%, respectively. Housekeeping genes groEL and gyrA were different between strain Cavy grass 6T and other streptococci. The G+C content of strain Cavy grass 6T was 42.6±0.3 mol%. The major (>10%) cellular fatty acids of strain Cavy grass 6T were C16:0, C20: 1ω9c and summed feature 8 (C18: 1ω7c and/or C18: 1ω6c). Strain Cavy grass 6T ferment a range of plant mono- and disaccharides as well as polymeric carbohydrates, including cellobiose, dulcitol, D-glucose, maltose, raffinose, sucrose, L-sorbose, trehalose, inulin and dried grass extract, to lactate, formate, acetate and ethanol. Based on phylogenetic and physiological characteristics, Cavy grass 6T can be distinguished from other members of the genus Streptococcus. Therefore, a novel species of the genus Streptococcus, family Streptococcaceae, order Lactobacillales is proposed, Streptococcus caviae sp. nov. (type strain Cavy grass 6T=TISTR 2371T=DSM 102819T).
KW - Cellobiose
KW - Dried grass
KW - Fermentation
KW - Inulin
KW - Lactic acid
KW - Streptococcus
U2 - 10.1099/ijsem.0.001758
DO - 10.1099/ijsem.0.001758
M3 - Article
AN - SCOPUS:85020471690
SN - 1466-5026
VL - 67
SP - 1551
EP - 1556
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 5
ER -