Streamlining genomes: toward the generation of simplified and stabilized microbial systems

A. Leprince, M.W.J. van Passel, V.A.P. Martins Dos Santos

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

At the junction between systems and synthetic biology, genome streamlining provides a solid foundation both for increased understanding of cellular circuitry, and for the tailoring of microbial chassis towards innovative biotechnological applications. Iterative genomic deletions (targeted and random) helps to generate simplified, stabilized and predictable genomes, whereas multiplexing genome engineering reveals a broad functional genetic diversity. The decrease in oligo and gene synthesis costs promises effective combinatorial tools for the generation of chassis based on streamlined and tractable genomes. Here we review recent progresses in streamlining genomes through recombineering techniques aiming to generate insights into cellular mechanisms and responses towards the design and assembly of streamlined genome chassis together with new cellular modules in diverse biotechnological applications
Original languageEnglish
Pages (from-to)651-658
JournalCurrent Opinion in Biotechnology
Volume23
Issue number5
DOIs
Publication statusPublished - 2012

Keywords

  • engineered escherichia-coli
  • reduced-genome
  • advanced biofuels
  • gene replacement
  • bacteria
  • design
  • expression
  • evolution
  • growth
  • host

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