Strategy to identify and quantify polysaccharide gums in gelled food concentrates

C.H. Grün, P. Sanders, M. van der Burg, E. Schuurbiers, L. van Adrichem, E.J.J. van Velzen, N. de Roo, K. Brunt, Y. Westphal, H.A. Schols

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

A strategy for the unambiguous identification and selective quantification of xanthan gum and locust bean gum (LBG) in gelled food concentrates is presented. DNA detection by polymerase chain reaction (PCR) showed to be a fast, sensitive, and selective method that can be used as a first screening tool in intact gelled food concentrates. An efficient isolation procedure is described removing components that may interfere with subsequent analyses. NMR spectroscopy enabled the direct identification of xanthan gum and the discrimination between different galactomannans in the isolated polysaccharide fraction. An enzymatic fingerprinting method using endo-ß-mannanase, in addition to being used to differentiate between galactomannans, was developed into a selective, quantitative method for LBG, whereas monosaccharide analysis was used to quantify xanthan gum. Recoveries for xanthan gum and LBG were 87% and 70%, respectively, with in-between day relative standard deviations below 20% for xanthan gum and below 10% for LBG.
Original languageEnglish
Pages (from-to)42-49
JournalFood Chemistry
Volume166
DOIs
Publication statusPublished - 2015

Keywords

  • locust bean gum
  • polymerase-chain-reaction
  • guar gum
  • capillary-electrophoresis
  • enzymatic determination
  • starch industry
  • raw-materials
  • xanthan gum
  • identification
  • additives

Fingerprint Dive into the research topics of 'Strategy to identify and quantify polysaccharide gums in gelled food concentrates'. Together they form a unique fingerprint.

  • Cite this

    Grün, C. H., Sanders, P., van der Burg, M., Schuurbiers, E., van Adrichem, L., van Velzen, E. J. J., de Roo, N., Brunt, K., Westphal, Y., & Schols, H. A. (2015). Strategy to identify and quantify polysaccharide gums in gelled food concentrates. Food Chemistry, 166, 42-49. https://doi.org/10.1016/j.foodchem.2014.05.129