Strategies for individual phenotyping of linoleic and arachidonic Acid metabolism using an oral glucose tolerance test

E. Saccenti, J.P.M. van Duynhoven, D.M. Jacobs, A.K. Smilde, H.C. Hoefsloot

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

The ability to restore homeostasis upon environmental challenges has been proposed as a measure for health. Metabolic profiling of plasma samples during the challenge response phase should offer a profound view on the flexibility of a phenotype to cope with daily stressors. Current data modeling approaches, however, struggle to extract biological descriptors from time-resolved metabolite profiles that are able to discriminate between different phenotypes. Thus, for the case of oxylipin responses in plasma upon an oral glucose tolerance test we developed a modeling approach that incorporates a priori biological pathway knowledge. The degradation pathways of arachidonic and linoleic acids were modeled using a regression model based on a pseudo-steady-state approximated model, resulting in a parameter A that summarizes the relative enzymatic activity in these pathways. Analysis of the phenotypic parameters As suggests that different phenotypes can be discriminated according to preferred relative activity of the arachidonic and linoleic pathway. Correlation analysis shows that there is little or no competition between the arachidonic and linoleic acid pathways, although they share the same enzymes
Original languageEnglish
Article numbere0119856
Number of pages16
JournalPLoS ONE
Volume10
Issue number3
DOIs
Publication statusPublished - 2015

Fingerprint

Linoleic Acid
Glucose Tolerance Test
arachidonic acid
Arachidonic Acid
Metabolism
linoleic acid
Oxylipins
Linoleic Acids
Arachidonic Acids
Phenotype
Plasmas
phenotype
Glucose
metabolism
Metabolites
Data structures
Health
oxylipins
Degradation
metabolomics

Keywords

  • challenges
  • pathways
  • biology
  • models
  • fat

Cite this

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title = "Strategies for individual phenotyping of linoleic and arachidonic Acid metabolism using an oral glucose tolerance test",
abstract = "The ability to restore homeostasis upon environmental challenges has been proposed as a measure for health. Metabolic profiling of plasma samples during the challenge response phase should offer a profound view on the flexibility of a phenotype to cope with daily stressors. Current data modeling approaches, however, struggle to extract biological descriptors from time-resolved metabolite profiles that are able to discriminate between different phenotypes. Thus, for the case of oxylipin responses in plasma upon an oral glucose tolerance test we developed a modeling approach that incorporates a priori biological pathway knowledge. The degradation pathways of arachidonic and linoleic acids were modeled using a regression model based on a pseudo-steady-state approximated model, resulting in a parameter A that summarizes the relative enzymatic activity in these pathways. Analysis of the phenotypic parameters As suggests that different phenotypes can be discriminated according to preferred relative activity of the arachidonic and linoleic pathway. Correlation analysis shows that there is little or no competition between the arachidonic and linoleic acid pathways, although they share the same enzymes",
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Strategies for individual phenotyping of linoleic and arachidonic Acid metabolism using an oral glucose tolerance test. / Saccenti, E.; van Duynhoven, J.P.M.; Jacobs, D.M.; Smilde, A.K.; Hoefsloot, H.C.

In: PLoS ONE, Vol. 10, No. 3, e0119856, 2015.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Strategies for individual phenotyping of linoleic and arachidonic Acid metabolism using an oral glucose tolerance test

AU - Saccenti, E.

AU - van Duynhoven, J.P.M.

AU - Jacobs, D.M.

AU - Smilde, A.K.

AU - Hoefsloot, H.C.

PY - 2015

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AB - The ability to restore homeostasis upon environmental challenges has been proposed as a measure for health. Metabolic profiling of plasma samples during the challenge response phase should offer a profound view on the flexibility of a phenotype to cope with daily stressors. Current data modeling approaches, however, struggle to extract biological descriptors from time-resolved metabolite profiles that are able to discriminate between different phenotypes. Thus, for the case of oxylipin responses in plasma upon an oral glucose tolerance test we developed a modeling approach that incorporates a priori biological pathway knowledge. The degradation pathways of arachidonic and linoleic acids were modeled using a regression model based on a pseudo-steady-state approximated model, resulting in a parameter A that summarizes the relative enzymatic activity in these pathways. Analysis of the phenotypic parameters As suggests that different phenotypes can be discriminated according to preferred relative activity of the arachidonic and linoleic pathway. Correlation analysis shows that there is little or no competition between the arachidonic and linoleic acid pathways, although they share the same enzymes

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