Strain-specific probiotic properties of bifidobacteria and lactobacilli for the prevention of diarrhea caused by rotavirus in a preclinical model

Ignasi Azagra-Boronat, Malén Massot-Cladera, Karen Knipping, Johan Garssen, Kaouther Ben Amor, Jan Knol, Àngels Franch, Margarida Castell, María J. Rodríguez-Lagunas*, Francisco J. Pérez-Cano

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Probiotic supplementation with different lactobacilli and bifidobacterial strains has demonstrated beneficial effects in infectious diarrhea caused by rotavirus (RV) in young children. Preclinical models of RV infection might be a good strategy to screen for the efficacy of new probiotic strains or to test their comparative efficacy. Neonatal Lewis rats were supplemented with Bifidobacterium breve M-16V, Lactobacillus acidophilus NCFM, Lactobacillus helveticus R0052, or Lactobacillus salivarius PS2 from days 2–14 of life. On day five, animals received RV SA-11 orally. Fecal samples were collected daily, weighed, and scored for the calculation of severity and incidence of diarrhea. In addition, fecal pH and fecal viral shedding were measured. Animals were sacrificed at the end of the study and their blood was obtained for the quantification of RV-specific immunoglobulins. RV infection was induced in ~90% of the animals. All probiotics caused a reduction of several clinical variables of severity and incidence of diarrhea, except L. salivarius PS2. L. acidophilus NCFM, B. breve M-16V, and L. helveticus R0052 seemed to be very effective probiotic strains. In addition, all Lactobacillus strains reduced the viral elimination one day post-inoculation. No differences were detected in the specific anti-RV humoral response. The present study highlights the strain-specific effects of probiotics and identifies promising probiotics for use in ameliorating and preventing RV-induced diarrhea in children, for example by including them in infant formulas.

Original languageEnglish
Article number498
JournalNutrients
Volume12
Issue number2
DOIs
Publication statusPublished - 1 Feb 2020

Keywords

  • Diarrhea
  • Probiotic
  • Rotavirus
  • Suckling rats

Fingerprint Dive into the research topics of 'Strain-specific probiotic properties of bifidobacteria and lactobacilli for the prevention of diarrhea caused by rotavirus in a preclinical model'. Together they form a unique fingerprint.

  • Cite this

    Azagra-Boronat, I., Massot-Cladera, M., Knipping, K., Garssen, J., Ben Amor, K., Knol, J., Franch, À., Castell, M., Rodríguez-Lagunas, M. J., & Pérez-Cano, F. J. (2020). Strain-specific probiotic properties of bifidobacteria and lactobacilli for the prevention of diarrhea caused by rotavirus in a preclinical model. Nutrients, 12(2), [498]. https://doi.org/10.3390/nu12020498