Stochastic gene expression in Arabidopsis thaliana

Ilka Schultheiß Araújo, Jessica Magdalena Pietsch, Emma Mathilde Keizer, Bettina Greese, Rachappa Balkunde, Christian Fleck*, Martin Hülskamp

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

Although plant development is highly reproducible, some stochasticity exists. This developmental stochasticity may be caused by noisy gene expression. Here we analyze the fluctuation of protein expression in Arabidopsis thaliana. Using the photoconvertible KikGR marker, we show that the protein expressions of individual cells fluctuate over time. A dual reporter system was used to study extrinsic and intrinsic noise of marker gene expression. We report that extrinsic noise is higher than intrinsic noise and that extrinsic noise in stomata is clearly lower in comparison to several other tissues/cell types. Finally, we show that cells are coupled with respect to stochastic protein expression in young leaves, hypocotyls and roots but not in mature leaves. Our data indicate that stochasticity of gene expression can vary between tissues/cell types and that it can be coupled in a non-cell-autonomous manner.
Original languageEnglish
Article number2132
JournalNature Communications
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017

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    Araújo, I. S., Pietsch, J. M., Keizer, E. M., Greese, B., Balkunde, R., Fleck, C., & Hülskamp, M. (2017). Stochastic gene expression in Arabidopsis thaliana. Nature Communications, 8(1), [2132]. https://doi.org/10.1038/s41467-017-02285-7